Myosin Antikörper (AA 12-27)

Produktnummer ABIN129691

Produktdetails anti-Myosin Antikörper

Target: Myosin

Bindungsspezifität: AA 12-27

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Myosin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP)

Kreuzreaktivität: Maus, Ratte (Rattus)

Produktmerkmale: Concentration Definition: by UV absorbance at 280 nm

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 12-27 of human myosin light chain protein.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: This affinity-purified antibody was tested by ELISA and immunoblotting and was found to be reactive with both the unphosphorylated and mono-phosphorylated forms of the protein.   Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.71 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Referenzen für anti-Myosin Antikörper (ABIN129691)

Deng, Bhaidani, Sutherland, MacDonald, Walsh et al.: "Rho-associated kinase and zipper-interacting protein kinase, but not myosin light chain kinase, are involved in the regulation of myosin phosphorylation in serum-stimulated human arterial smooth ..." in: PLoS ONE, Vol. 14, Issue 12, pp. e0226406, (2020) (PubMed).

Lahey, Ronaghan, Shang, Dion, Désilets, Leduc, MacNaughton: "Signaling pathways induced by serine proteases to increase intestinal epithelial barrier function." in: PLoS ONE, Vol. 12, Issue 7, pp. e0180259, (2017) (PubMed).

Logue, Cartagena-Rivera, Baird, Davidson, Chadwick, Waterman: "Erk regulation of actin capping and bundling by Eps8 promotes cortex tension and leader bleb-based migration." in: eLife, Vol. 4, pp. e08314, (2016) (PubMed).

Details zu Myosin

Target: Myosin

Abstract: Myosin Produkte

Synonyme: CG3201 antikoerper, DmMLC_C antikoerper, Dmel\\CG3201 antikoerper, MyoII antikoerper, mlc-c antikoerper, nmELC antikoerper, MYOSIN antikoerper, myosin 1 antikoerper, DFNA4 antikoerper, DFNA4A antikoerper, MHC16 antikoerper, MYH17 antikoerper, NMHC II-C antikoerper, NMHC-II-C antikoerper, PNMHH antikoerper, myosin antikoerper, MYH1 antikoerper, MYH2 antikoerper, MYH3 antikoerper, MYH4 antikoerper, MYH8 antikoerper, adult antikoerper, embryonic antikoerper, 2400004E04Rik antikoerper, II-C antikoerper, NHMCII antikoerper, DROMHC antikoerper, Dm nmII antikoerper, DmnmII antikoerper, Dronm-MII antikoerper, E(br) antikoerper, MHC antikoerper, Mhc-c antikoerper, Mhc-c[1] antikoerper, Myo antikoerper, Myo II antikoerper, NMM antikoerper, Zip antikoerper, anon-WO0140519.37 antikoerper, l(2)02957 antikoerper, nmMHC antikoerper, nmy-2 antikoerper, zpr antikoerper, DmelCG15792 antikoerper, CG15792 antikoerper, Myosin light chain cytoplasmic antikoerper, hypothetical protein antikoerper, myosin 1 antikoerper, myosin heavy chain 9 antikoerper, myosin heavy chain 14 antikoerper, myosin, heavy chain 1B, skeletal muscle antikoerper, myosin, heavy polypeptide 14 antikoerper, zipper antikoerper, Mlc-c antikoerper, T22C1.6 antikoerper, ATM1 antikoerper, MYH9 antikoerper, MYH14 antikoerper, Myh14 antikoerper, MYH1B antikoerper, zip antikoerper

Hintergrund: Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail.  The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons.  Activation of smooth and cardiac muscle primarily involves pathways that increase calcium and myosin phosphorylation resulting in contraction.   Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain.  The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells.  This sequence differs from that of the sarcomeric/
Synonyms: Myosin regulatory light polypeptide 9 Myosin regulatory light chain 9 Myosin regulatory light chain MRLC1 Myosin regulatory light chain 2, smooth muscle isoform Myosin RLC MLC-2C 20 kDa myosin light chain

Gen-ID: 98932, 38605043

UniProt: P24844