PDCD4 Antikörper (C-Term)

Produktnummer ABIN129709

Hersteller Produkt- Nr.: 600-401-965

Produktdetails anti-PDCD4 Antikörper

Target: PDCD4 (Programmed Cell Death 4 (PDCD4))

Bindungsspezifität: C-Term

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PDCD4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Hersteller Produkt- Nr.: 600-401-965

Hersteller: Rockland

Verwendungszweck: Pdcd4 Antibody

Kreuzreaktivität (Details): This affinity purified antibody is directed against human Pdcd4 protein.

Produktmerkmale: Synonyms: rabbit anti-PDCD4 antibody, PDCD-4, PDCD 4, Programmed cell death protein 4, Death up-regulated gene protein antibody, Dug antibody, H731 antibody, Ma3 antibody, Neoplastic transformation inhibitor antibody, Neoplastic transformation inhibitor protein antibody, Nuclear antigen H731 antibody, Protein 197/15a

Aufreinigung: The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Sterilität: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding amino acids near the carboxyl terminus of human Pdcd4 protein.

Immunogen Type: Conjugated Peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:100 - 1:300

Application Note: This affinity purified antibody has been tested for use in ELISA, western blotting, immunoprecipitation and immunohistochemistry.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 52 kDa in size corresponding to Pdcd4 protein by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:1,000 - 1:10,000

ELISA Dilution: 1:10,000 - 1:100,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1.06 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-PDCD4 Antikörper (ABIN129709)

Hill, Nesser, Johnson-Camacho, Jeffress, Johnson, Boniface, Spencer, Lu, Heiser, Lawrence, Pande, Korkola, Gray, Mills, Mukherjee, Spellman: "Context Specificity in Causal Signaling Networks Revealed by Phosphoprotein Profiling." in: Cell systems, Vol. 4, Issue 1, pp. 73-83.e10, (2019) (PubMed).

Kim, Hu, Jadhav, Jin, Zhang, Cavanagh, Akondy, Ahmed, Weyand, Goronzy: "Activation of miR-21-Regulated Pathways in Immune Aging Selects against Signatures Characteristic of Memory T Cells." in: Cell reports, Vol. 25, Issue 8, pp. 2148-2162.e5, (2018) (PubMed).

Liwak-Muir, Dobson, Naing, Wylie, Chehade, Baird, Chakraborty, Holcik: "ERK8 is a novel HuR kinase that regulates tumour suppressor PDCD4 through a miR-21 dependent mechanism." in: Oncotarget, Vol. 7, Issue 2, pp. 1439-50, (2016) (PubMed).

Sharma, Lin, Farrugia, McLaughlin, Ellis, Brundage, Salkeni, Ruppert: "MicroRNAs 206 and 21 cooperate to promote RAS-extracellular signal-regulated kinase signaling by suppressing the translation of RASA1 and SPRED1." in: Molecular and cellular biology, Vol. 34, Issue 22, pp. 4143-64, (2015) (PubMed).

Yang, Yue, Sims, Pfeffer: "The curcumin analog EF24 targets NF-κB and miRNA-21, and has potent anticancer activity in vitro and in vivo." in: PLoS ONE, Vol. 8, Issue 8, pp. e71130, (2014) (PubMed).

Hirata, Suzuki, Imaeda, Matsuzaki, Tsugawa, Nagano, Asakura, Saya, Hibi: "CD44 variant 9 expression in primary early gastric cancer as a predictive marker for recurrence." in: British journal of cancer, Vol. 109, Issue 2, pp. 379-86, (2013) (PubMed).

Ruan, Wang, Kameswaran, Wei, Johnson, Matschinsky, Shi, Chen: "The microRNA-21-PDCD4 axis prevents type 1 diabetes by blocking pancreatic beta cell death." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 29, pp. 12030-5, (2011) (PubMed).

Santhanam, Baker, Hegamyer, Kirschmann, Colburn: "Pdcd4 repression of lysyl oxidase inhibits hypoxia-induced breast cancer cell invasion." in: Oncogene, Vol. 29, Issue 27, pp. 3921-32, (2010) (PubMed).

OHayre, Salanga, Kipps, Messmer, Dorrestein, Handel: "Elucidating the CXCL12/CXCR4 Signaling Network in Chronic Lymphocytic Leukemia through Phosphoproteomics Analysis." in: PLoS ONE, Vol. 5, Issue 7, pp. e11716, (2010) (PubMed).

Huang, Wu, Loeb, Hsu, Heidersbach, Brincat, Horiuchi, Lebbink, Mo, Goga, McManus: "Up-regulation of miR-21 by HER2/neu signaling promotes cell invasion." in: The Journal of biological chemistry, Vol. 284, Issue 27, pp. 18515-24, (2009) (PubMed).

Jansen, Camalier, Colburn: "Epidermal expression of the translation inhibitor programmed cell death 4 suppresses tumorigenesis." in: Cancer research, Vol. 65, Issue 14, pp. 6034-41, (2005) (PubMed).

Details zu PDCD4

Target: PDCD4 (Programmed Cell Death 4 (PDCD4))

Andere Bezeichnung: PDCD4 (PDCD4 Produkte)

Hintergrund: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Programmed cell death 4 (Pdcd4) is a novel tumor suppressor. Pdcd4 directly inhibits the helicase activity of eukaryotic translation initiation factor 4A (eIF4A), a component of the translation initiation complex. Pdcd4 also suppresses the transactivation of activator protein-1 (AP-1)-responsive promoters by c-Jun. Pdcd4 contains two Akt phosphorylation sites, one at Ser67 and the other at Ser457. The phosphorylation of Pdcd4 by Akt causes nuclear translocation of Pdcd4 and a significant decrease in the ability of Pdcd4 to interfere with the transactivation of AP-1-responsive promoters by c-Jun.

Gen-ID: 27250, 21735596

UniProt: Q53EL6