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RNF25 Antikörper (C-Term)

Rockland 600-401-969 RNF25 Reaktivität: Human, Maus WB, ELISA, IP Wirt: Kaninchen Polyclonal unconjugated
Rockland
Produktnummer ABIN129713
Hersteller Produkt- Nr.: 600-401-969
  • Target Alle RNF25 Antikörper anzeigen
    RNF25 (Ring Finger Protein 25 (RNF25))
    Bindungsspezifität
    • 15
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reaktivität
    • 29
    • 17
    • 17
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Maus
    Wirt
    • 25
    • 4
    Kaninchen
    Klonalität
    • 27
    • 2
    Polyklonal
    Konjugat
    • 15
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser RNF25 Antikörper ist unkonjugiert
    Applikation
    • 29
    • 13
    • 7
    • 6
    • 3
    • 2
    • 2
    • 1
    Western Blotting (WB), ELISA, Immunoprecipitation (IP)
    Hersteller Produkt- Nr.
    600-401-969
    Hersteller
    Rockland
    Verwendungszweck
    RNF25 Antibody
    Kreuzreaktivität (Details)
    This affinity purified antibody is directed against human RNF25 protein.
    Produktmerkmale
    Synonyms: rabbit anti-Rnf25 antibody, E3 ubiquitin protein ligase RNF25 antibody, Ring finger protein 25 antibody, RING-type E3 ubiquitin transferase Rnf25 antibody, RNF 25, RNF-25
    Aufreinigung
    The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
    Sterilität
    Sterile filtered
    Immunogen

    Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the carboxyl terminus of human RNF25 protein.

    Immunogen Type: Conjugated Peptide

    Isotyp
    IgG
    Top Product
    Discover our top product RNF25 Primärantikörper
  • Applikationshinweise

    Application Note: This affinity purified antibody has been tested for use in ELISA, western blot and immunoprecipitation.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 54 kDa in size corresponding to RNF25 protein by western blotting in the appropriate cell lysate or extract.

    Western Blot Dilution: 1:200 - 1:2,000

    Immunoprecipitation Dilution: 1 mg

    ELISA Dilution: 1:10,000 - 1:40,000

    Other: User Optimized

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1.0 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: None

    Preservative: 0.01 % (w/v) Sodium Azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Haltbarkeit
    12 months
  • Target
    RNF25 (Ring Finger Protein 25 (RNF25))
    Andere Bezeichnung
    RNF25 (RNF25 Produkte)
    Hintergrund
    Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. RING finger protein 25 (RNF25, also named AO7) contains a RING finger domain and is ubiquitously expressed in various tissues.  RNF25 was initially identified in a yeast two-hybrid screen of a murine T-cell library by using UbcH5b, an E2 enzyme, as bait.  RNF25 has also been shown to act as a putative E3 ligase, at least in vitro.  RNF25 localizes predominantly in the nucleus and supports the transcriptional activity of NF-kB by interacting with p65 in vivo upon stimulation with TNF.  Yeast two-hybrid data also suggest that RNF25 interacts with a number of other molecules which may be potential ubiquitin ligase substrates.  Among these are molecules that have critical roles in signal transduction and in regulation of translation (personal communication, Allan Weissman, CCR-NCI, Bethesda, MD).
    Gen-ID
    64320, 34878787
    UniProt
    Q96BH1
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