Nitrotyrosine Antikörper
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- Target Alle Nitrotyrosine Antikörper anzeigen
- Nitrotyrosine
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Reaktivität
- Diverse Spezies
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser Nitrotyrosine Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Spezifität
- The antibody EM-30 detects nitrotyrosine group in biological material. It can be used as a marker for peroxynitrite formation in particular tissues.
- Kreuzreaktivität (Details)
- Broad
- Aufreinigung
- Purified by protein-A affinity chromatography.
- Reinheit
- > 95 % (by SDS-PAGE)
- Immunogen
- NO2-Tyr-CH2-Thyroglobulin
- Klon
- EM-30
- Isotyp
- IgG1
- Top Product
- Discover our top product Nitrotyrosine Primärantikörper
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- Applikationshinweise
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Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL, positive tissue: colon cancer epithelium, prostate hyperplasia.
Immunohistochemistry (frozen sections): Recommended dilution: 10 μg/mL, positive tissue: colon cancer epithelium, prostate hyperplasia.
Western blotting: positive control: NO2-BSA, NO2-HSA. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 1 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Do not freeze.
- Lagerung
- 4 °C
- Informationen zur Lagerung
- Store at 2-8°C. Do not freeze.
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- Target
- Nitrotyrosine
- Abstract
- Nitrotyrosine Produkte
- Substanzklasse
- Chemical
- Hintergrund
- Nitrotyrosine can be detected in proteins from a variety of tissues usually in association with pathological conditions. Reaction of nitric oxide with superoxide produces peroxynitrite, which can undergo heterolytic cleavage into nitronium and hydroxyl ions. Nitration of tyrosine residues by nitronium ion forms nitrotyrosine groups in the respective proteins. Nitrotyrosine is thus a marker for inflammation-associated tissue damage.,TyrNO2, C9H10N2O5
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