Working Dilutions: Flow Cytometry FITC and BIOT conjugates 1 g/106 cells PE conjugate 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
Probenmenge
1 mL
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
0.5 mg/mL
Buffer
0.5 mg of purified immunoglobulin in 1.0 mL of borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added
Konservierungsmittel
Without preservative
Handhabung
Each reagent is stable for the period shown on the bottle label if stored as directed.
CD45 antikoerper, L-CA antikoerper, Lca antikoerper, RT7 antikoerper, T200 antikoerper, protein tyrosine phosphatase, receptor type, C antikoerper, Ptprc antikoerper
Hintergrund
The CD45 family of cell-surface glycoprotein antigens exist as a set of isoforms. The patterns of monoclonal antibody recognition of the CD45 phenotypes can be restricted (CD45RA, RB and RC), or broader, such as the CD45 T-200 species. The restricted subsets of this group arise as a result of translation of alternatively spliced mRNAs from exons A, B and C (exons 4, 5 and 6), where exons 3-15 encode the extracellular domain of the CD45 molecule. The restricted epitopes are differentially expressed on T and B lymphocytes, including functionally distinct T cells.