Dieser MHC Class II Antikörper ist konjugiert mit FITC
Applikation
Flow Cytometry (FACS)
Spezifität
Chicken/Pigeon/Caiman MHC Class II β-chain, Mr 30-42 kDa
Produktmerkmale
Mouse Anti-Chicken MHC Class II-FITC
Aufreinigung
The antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide is removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
Applications: FC - Quality tested , IHC-FS - Reported in literature , EM - Reported in literature , IP - Reported in literature , Purification - Reported in literature
Working Dilutions: Flow Cytometry Purified (UNLB) antibody 1 g/106 cells FITC, BIOT, and AF488 conjugates 1 g/106 cells PE conjugate 0.2 g/106 cells For flow cytometry, the suggested use of these reagents is in a final volume of 100 L
Probenmenge
1 mL
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
0.5 mg/mL
Buffer
0.5 mg in 1.0 mL of PBS/Sodium azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Protect conjugated products from light. Each reagent is stable for the period shown on the bottle label if stored as directed.
Lagerung
4 °C
Informationen zur Lagerung
Store at 2-8°C
Target
MHC Class II (MHC2)
(Major Histocompatibility Complex Class II Antigen (MHC2))
The chicken major histocompatibility complex (MHC), or B complex, consists of several clusters of highly polymorphic genes. Like their mammalian counterparts, the avian MHC exerts genetic influence over a variety of important biological functions such as immune response, disease resistance, growth and development, aging, and reproduction. Chicken MHC Class II genes, also known as the B-L subregion, of the chicken MHC encode cell surface glycoproteins that are homologous to mammalian Class II antigens. B-L antigens structurally are similar to mammalian Class II molecules in that they are noncovalently bound dimmers of one 32-34 kDa heavy chain and one 27-30 kDa light chain. MHC Class II is primarily expressed on B cells and antigen presenting cells (APCs).