DPH2 Antikörper (AA 401-489)

Produktnummer ABIN1385915

Der Kaninchen Polyklonal Anti-DPH2-Antikörper wurde für WB, ELISA, IHC (p), IF (cc), IF (p) und IHC (fro) validiert. Er ist geeignet, DPH2 in Proben von Human zu detektieren.

Kurzübersicht für DPH2 Antikörper (AA 401-489) (ABIN1385915)

Target: DPH2 (Diphthamide Biosynthesis Protein 2 (DPH2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DPH2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-DPH2 Antikörper

Bindungsspezifität: AA 401-489

Kreuzreaktivität: Human

Homologie: Mouse,Rat,Dog,Cow,Sheep,Horse,Chicken,Rabbit

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human DPH2

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu DPH2

Target: DPH2 (Diphthamide Biosynthesis Protein 2 (DPH2))

Andere Bezeichnung: Dph2

Hintergrund:

Synonyms: Diphthamide biosynthesis like protein 2, Diphthamide biosynthesis protein 2, Diphthamide biosynthesis protein 2 homolog like 2, Diphthamide biosynthesis protein 2 homolog-like 2, Diptheria toxin resistance protein required for diphthamide biosynthesis like 2, Diptheria toxin resistance protein required for diphthamide biosynthesis like 2 S. cerevisiae , DPH 2, DPH2, DPH2 homolog, DPH2 homolog S. cerevisiae, DPH2 like 2, DPH2 like 2 S. cerevisiae, DPH2-like 2, DPH2_HUMAN, DPH2L2, HsDph2, OTTHUMP00000010007, Protein DPH2 homolog.

Background: DPH2 (diphthamide biosynthesis protein 2), also known as DPH2L2, is a 489 amino acid protein that shows strong expression in skeletal muscle, moderate expression in heart, small intestine, liver, pancreas, testis and colon, and lesser expression in brain, placenta, kidney, spleen, thymus, prostate, ovary and lymphocytes. DPH2 interacts with DPH1 and, functioning together as a dimer or multimer, DPH1 and DPH2 may participate in diphthamide biosynthesis. Diphthamide is a posttranslationally modified histidine residue which occurs in EF-2 (elongation factor 2) and targets diphtheria toxin ADP-ribosylation. The loss of DPH2 in Saccharomyces cerevisiae is believed to suppress zymocicity. Two transcript variants encoding different isoforms have been found for this gene.Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2.Tissue specificity:Strongly expressed in skeletal muscle. Moderately expressed in heart, small intestine, liver, pancreas, testis and colon. Weakly expressed in brain, placenta, kidney, speen, thymus, prostate, ovary and lymphocytes.

Gen-ID: 1802