AKT 1/2/3 Antikörper (pTyr474)
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- Target Alle AKT 1/2/3 Produkte
- AKT 1/2/3
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Bindungsspezifität
- pTyr474
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Reaktivität
- Human, Maus
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser AKT 1/2/3 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Flow Cytometry (FACS), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)
- Spezifität
- This antibody detects Akt1 when phosphorylated at Tyr474 and correspondingly, Akt2 when phosphorylated at Tyr 475 and Akt3 when phosphorylated at Tyr473
- Kreuzreaktivität
- Human, Maus
- Homologie
- Rat,Dog,Cow,Sheep,Pig,Chicken,Rabbit
- Aufreinigung
- Purified by Protein A.
- Immunogen
- KLH conjugated synthetic phosphopeptide derived from human AKT1 around the phosphorylation site of Tyr474
- Isotyp
- IgG
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anti-AKT 1/2/3 (AA 401-479) antibody
Reaktivität: Human, Maus, Ratte, Rind (Kuh), Schwein WB, IF (cc), IF (p), FACS, ELISA, IHC (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated
anti-AKT 1/2/3 (pSer473) antibodyReaktivität: Human, Maus, Ratte, Rind (Kuh), Kaninchen, Huhn, Fisch WB, IF (cc), IF (p), ELISA, IHC (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated
anti-AKT 1/2/3 antibodyReaktivität: Human WB, IHC, IF, IP Wirt: Kaninchen Polyclonal unconjugated
anti-AKT 1/2/3 (pSer472) antibodyReaktivität: Human WB, IHC Wirt: Kaninchen Polyclonal unconjugated
anti-AKT 1/2/3 (pTyr312), (pTyr315), (pTyr316) antibodyReaktivität: Human, Maus, Ratte WB, IF (cc), IF (p), FACS, ELISA, IHC (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated
anti-AKT 1/2/3 (pTyr312), (pTyr315), (pTyr316) antibodyReaktivität: Human, Maus, Ratte WB, IHC, IF, ICC Wirt: Kaninchen Polyclonal unconjugated
anti-AKT 1/2/3 (pThr305), (pThr308), (pThr309) antibodyReaktivität: Human, Maus, Ratte WB, IF (cc), IF (p), FACS, ELISA, IHC (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated
anti-AKT 1/2/3 (AA 278-312) antibodyReaktivität: Human, Ratte WB, IHC (p) Wirt: Kaninchen Polyclonal RB43841 unconjugated
anti-AKT 1/2/3 (AA 431-480) antibodyReaktivität: Human, Maus, Ratte WB, IHC, ELISA Wirt: Kaninchen Polyclonal unconjugated
anti-AKT 1/2/3 (pSer472) antibodyReaktivität: Human WB Wirt: Kaninchen Monoclonal unconjugated
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- Applikationshinweise
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WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500 - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 1 μg/μL
- Buffer
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- Konservierungsmittel
- ProClin
- Vorsichtsmaßnahmen
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Haltbarkeit
- 12 months
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- Target
- AKT 1/2/3
- Andere Bezeichnung
- AKT1/2/3 (AKT 1/2/3 Produkte)
- Hintergrund
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Synonyms: RAC-alpha serine/threonine-protein kinase, Protein kinase B, PKB, Protein kinase B alpha, PKB alpha, Proto-oncogene c-Akt, RAC-PK-alpha, AKT1, RAC
Background: AKT1-specific substrates have been recently identified, including palladin (PALLD), which phosphorylation modulates cytoskeletal organization and cell motility, prohibitin (PHB), playing an important role in cell metabolism and proliferation, and CDKN1A, for which phosphorylation at 'Thr-145' induces its release from CDK2 and cytoplasmic relocalization. These recent findings indicate that the AKT1 isoform has a more specific role in cell motility and proliferation. Phosphorylates CLK2 thereby controlling cell survival to ionizing radiation.
- Gen-ID
- 207
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