UBE2D3 Antikörper (AA 55-147)

Produktnummer ABIN1387890

Produktdetails anti-UBE2D3 Antikörper

Target: UBE2D3 (Ubiquitin-Conjugating Enzyme E2D 3 (UBE2D3))

Bindungsspezifität: AA 55-147

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser UBE2D3 Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Kreuzreaktivität: Maus

Homologie: Human,Rat,Cow,Pig,Horse,Chicken,Rabbit,Zebrafish

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human UBE2D3

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu UBE2D3

Target: UBE2D3 (Ubiquitin-Conjugating Enzyme E2D 3 (UBE2D3))

Andere Bezeichnung: UBE2D3 (UBE2D3 Produkte)

Synonyme: E2(17)KB3 antikoerper, UBC4/5 antikoerper, UBCH5C antikoerper, eff antikoerper, fb36f02 antikoerper, wu:fb36f02 antikoerper, zgc:56340 antikoerper, 1100001F19Rik antikoerper, 9430029A22Rik antikoerper, AA414951 antikoerper, UBE2D3P antikoerper, Xubc4 antikoerper, e2(17)kb3 antikoerper, ubc4/5 antikoerper, ubch5c antikoerper, ube2d3.1 antikoerper, ube2d2 antikoerper, xubc4 antikoerper, wu:fj13d01 antikoerper, zgc:73200 antikoerper, ubiquitin conjugating enzyme E2 D3 antikoerper, ubiquitin-conjugating enzyme E2D 3 antikoerper, ubiquitin conjugating enzyme E2 D3 L homeolog antikoerper, ubiquitin-conjugating enzyme E2D 2 (UBC4/5 homolog, yeast), like antikoerper, UBE2D3 antikoerper, ube2d3 antikoerper, Ube2d3 antikoerper, LOC100223566 antikoerper, ube2d3.L antikoerper, ube2d2l antikoerper

Hintergrund:

Synonyms: E217KB3, MGC43926, MGC5416, UB2D3_HUMAN, UBC 4/5, UBC4/5, UBC4/5 homolog yeast, UBCH 5C, UBCH5C, Ube2d3, Ubiquitin carrier protein, Ubiquitin carrier protein D3, Ubiquitin conjugating enzyme E2 17 kDa 3, Ubiquitin conjugating enzyme E2 D3, Ubiquitin conjugating enzyme E2D 3 homologous to yeast UBC4/5, Ubiquitin conjugating enzyme E2D 3 UBC4/5 homolog yeast, Ubiquitin conjugating enzyme E2D 3, Ubiquitin protein ligase D3, Ubiquitin-conjugating enzyme E2 D3, Ubiquitin-conjugating enzyme E217KB 3, Ubiquitin-conjugating enzyme E2-17 kDa 3, Ubiquitin-protein ligase D3.

Background: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'-linked polyubiquitination. Cooperates with the E2 CDC34 and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Ubiquitin chain elongation is then performed by CDC34, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. Acts also as an initiator E2, in conjunction with RNF8, for the priming of PCNA. Monoubiquitination of PCNA, and its subsequent polyubiquitination, are essential events in the operation of the DNA damage tolerance (DDT) pathway that is activated after DNA damage caused by UV or chemical agents during S-phase. Associates with the BRCA1/BARD1 E3 ligase complex to perform ubiquitination at DNA damage sites following ionizing radiation leading to DNA repair. Targets DAPK3 for ubiquitination which influences promyelocytic leukemia protein nuclear body (PML-NB) formation in the nucleus. In conjunction with the MDM2 and TOPORS E3 ligases, functions ubiquitination of p53/TP53. Supports NRDP1-mediated ubiquitination and degradation of ERBB3 and of BRUCE which triggers apoptosis. In conjunction with the CBL E3 ligase, targets EGFR for polyubiquitination at the plasma membrane as well as during its internalization and transport on endosomes. In conjunction with the STUB1 E3 quality control E3 ligase, ubiquitinates unfolded proteins to catalyze their immediate destruction.

Gen-ID: 7323

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades