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CD8 alpha Antikörper (AA 51-150)

Name: CD8 alpha Antikörper (AA 51-150)
SKU: ABIN1387948
Price: 384.62 EUR
Availability: InStock

CD8A Reaktivität: Human, Ratte, Huhn FACS, WB, ELISA, IHC (p), IHC (fro), IF (p), IF (cc) Wirt: Kaninchen Polyclonal unconjugated

Paraformaldehyde-fixed, paraffin embedded rat spleen, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with CD8 alpha Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining. (CD8 alpha Antikörper (AA 51-150))

IHC (p)

Formalin-fixed and paraffin embedded rat colon labeled with Anti-CD8 alpha Polyclonal Antibody, Unconjugated (ABIN1387948) at 1:200 followed by secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C. (CD8 alpha Antikörper (AA 51-150))

IF (p)

3 Abbildungen

(2 Referenzen)

Produktnummer ABIN1387948

Datenblatt (PDF)

Target Alle CD8 alpha (CD8A) Antikörper anzeigen

CD8 alpha (CD8A) (CD8a Molecule (CD8A))
Bindungsspezifität
15

15

10

9

9

9

8

7

6

6

5

5

3

3

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 51-150
Reaktivität
205

117

46

44

15

7

3

2

2

1

1

1

1

1

1

1

1
Human, Ratte, Huhn
Wirt
174

126

104
Kaninchen
Passende Sekundärantikörper
Klonalität
298

102

1
Polyklonal
Konjugat
118

46

36

30

30

11

9

8

8

8

8

7

6

6

5

5

4

4

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser CD8 alpha Antikörper ist unkonjugiert
Applikation
310

129

92

67

65

46

21

18

14

14

13

11

10

8

7

7

6

6

5

3

3

3

2

1

1

1

1

1

1

1

1

1

1

1
Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Kreuzreaktivität

Huhn, Human, Ratte

Aufreinigung

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from rat CD8 alpha

Isotyp

IgG

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CD8A Reaktivität: Maus FACS Wirt: Ratte Monoclonal 53-6-7 FITC

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CD8A Reaktivität: Human FACS, IHC, WB, ELISA Wirt: Kaninchen Polyclonal unconjugated

Applikationshinweise

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel

ProClin

Vorsichtsmaßnahmen

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit

12 months
Savvatis, Müller, Fröhlich, Pappritz, Zietsch, Hamdani, Grote, Schieffer, Klingel, Van Linthout, Linke, Schultheiss, Tschöpe: "Interleukin-6 receptor inhibition modulates the immune reaction and restores titin phosphorylation in experimental myocarditis." in: Basic research in cardiology, Vol. 109, Issue 6, pp. 449, (2014) (PubMed).

Cheng, Guo, Qiao, Liu, Nie, Li, Wang, Jiang: "T cell immunohistochemistry refines lung transplant acute rejection diagnosis and grading." in: Diagnostic pathology, Vol. 8, pp. 168, (2013) (PubMed).
Target

CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Andere Bezeichnung

CD8 alpha (CD8A Produkte)

Synonyme

cd8 antikoerper, LOC100136450 antikoerper, BB154331 antikoerper, Ly-2 antikoerper, Ly-35 antikoerper, Ly-B antikoerper, Lyt-2 antikoerper, zgc:136643 antikoerper, CD8 antikoerper, Leu2 antikoerper, MAL antikoerper, p32 antikoerper, RHACD8-4 antikoerper, CD8a molecule antikoerper, uncharacterized LOC100125537 antikoerper, CD8 alpha antikoerper, CD8 antigen, alpha chain antikoerper, T-cell surface glycoprotein CD8 alpha chain antikoerper, Cd8a antikoerper, LOC100125537 antikoerper, LOC100136450 antikoerper, CD8A antikoerper, cd8a antikoerper, LOC100126556 antikoerper

Hintergrund

Synonyms: T-cell surface glycoprotein CD8 alpha chain, CD8 antigen 32 kDa chain, OX-8 membrane antigen, CD8a
Background: Identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD8 is thought to play a role in the process of T-cell mediated killing. CD8 alpha chains binds to class I MHC molecules alpha-3 domains.

Gen-ID

24930

UniProt

P07725

Pathways

T-Zell Rezeptor Signalweg