AAV VP1 Antikörper (AbBy Fluor® 647)

Produktnummer ABIN1388676

Produktdetails anti-AAV VP1 Antikörper (AbBy Fluor® 647)

Target: AAV VP1 (Adeno-Associated Virus, VP1 (AAV VP1))

Reaktivität: Adeno-Associated Virus (AAV)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser AAV VP1 Antikörper ist konjugiert mit AbBy Fluor® 647

Applikation: Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Spezifität: This antibody will recognize many AAV strains, including AAV5, AAV4, AAV3B, AAV9, and AAV13

Kreuzreaktivität: Virus

Kreuzreaktivität (Details): AAV5

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from Adeno-Associated Virus 5 capsid protein VP1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: IF(IHC-P) 1:50-200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu AAV VP1

Target: AAV VP1 (Adeno-Associated Virus, VP1 (AAV VP1))

Andere Bezeichnung: Adeno-Associated Virus Capsid Protein VP1 (AAV VP1 Produkte)

Substanzklasse: Viral Protein

Hintergrund:

Synonyms: capsid protein Adeno-associated 5 virus, capsid protein [Adeno-associated virus - 5], capsid protein AAV5, Parvovirus coat protein VP1, capsid protein.

Background: Capsid protein self-assembles to form an icosahedral capsid with a T=1 symmetry, about 22 nm in diameter, and consisting of 60 copies of three size variants of the capsid protein VP1, VP2 and VP3 which differ in their N-terminus. The capsid encapsulates the genomic ssDNA. Binds to host cell heparan sulfate and uses host ITGA5-ITGB1 as coreceptor on the cell surface to provide virion attachment to target cell. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis. Binding to the host receptor also induces capsid rearrangements leading to surface exposure of VP1 N-terminus, specifically its phospholipase A2-like region and putative nuclear localization signal(s). VP1 N-terminus might serve as a lipolytic enzyme to breach the endosomal membrane during entry into host cell and might contribute to virus transport to the nucleus