Ammonium sulfate precipitation and affinity chromatography on protein A agarose
Immunogen
Recombinant E. coli RecA protein
Klon
ARM193
Isotyp
IgG2b
Applikationshinweise
Optimal working dilution should be determined by the investigator.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Rekonstitution
Prepare a stock solution by dissolving the lyophilized antibody in 100 μL of distilled water.
Konzentration
1.0 mg/mL (following reconstitution)
Buffer
PBS ( pH 7.2), 0.09 % NaN3, 1 % sucrose
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted in aliquots at -20 °C.
Target
Recombinase A / RecA
Hintergrund
RecA protein (38 kDa) is important in various cellular functions such as DNA repair, genetic recombination, induction of prophages, mutagenesis, cell division, and DNA replication in E. coli. RecA has DNA-dependent ATPase activity, and promotes ATP-dependent pairing of double stranded DNA and homologous single stranded fragments to form D-loops.Synonyms: lexB, recH, rnmB, tif, umuB, zab