This antibody stains normal colon and colonic carcinomas where it produces intense perinuclear staining in goblet cells. It also reacts with normal and neoplastic breast tissues and with prostate carcinoma. Cellular localization: Cytoplasmic, perinuclear staining of goblet cells.
Kreuzreaktivität (Details)
Species reactivity (tested):Human, Mouse and Rat.
Aufreinigung
Protein A Chromatography.
Immunogen
Synthetic MUC2 (23 aa) tandem repeat consensus sequence.
Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-FixedParaffin-Embedded (FFPE) tissue sections and cell smears). For IHC dilute concentared antibody at 1/50-1/200 using streptavidin-Biotin system orpolymer system. For FFPE tissue sections high temperature antigen retriever (boiling tissue in 10 mM Citratebuffer for 10 min) enhances the signal and may require further dilution of antibody. Positive Control: Human colon carcinoma, LS 174 T colon cancer cell line. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
2.0 mg/mL
Buffer
10 mM PO4, 200 mM NaCl, pH 7.4 containing 0.05 % Sodium Azide as preservative and 1 % BSA as stabilizer.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Mucin 2, oligomeric mucus/gel-forming, also known as MUC2, is a human intestinal mucin gene. This gene is expressed in colon, small intestine, colonic tumors, bronchus, cervix, gall bladder, and possibly other tissues. Mucins are high molecular weight glycoproteins having oligosaccharides attached to serine or threonine residue of the apomucin protein backbone by O-glycosidic linkages.Synonyms: Intestinal mucin-2, MUC-2, MUC2, SMUC