beta-2 Microglobulin Antikörper
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- Target Alle beta-2 Microglobulin (B2M) Antikörper anzeigen
- beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))
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Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser beta-2 Microglobulin Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA)
- Marke
- IHC-plus™
- Spezifität
- Reacts with beta2-microglobulin (b2M) associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta2-microglobulin. b2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes,thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes.
- Keine Kreuzreaktivität
- Maus, Rind (Kuh), Kaninchen, Huhn, Hund
- Aufreinigung
- Purified
- Immunogen
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Purified human beta2-microglobulin
Type of Immunogen: Purified protein - Klon
- B2M-01
- Isotyp
- IgG2a
- Top Product
- Discover our top product B2M Primärantikörper
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- Applikationshinweise
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Approved: ELISA, Flo, IHC, IHC-P (1:50), IP, RIA, WB (2 - 4 μg/mL)
Usage: For use in immunoprecipitation, this antibody coprecipitates b2-microglobulin (12 kDa) and HLA antigens(45 kDa) from the lysates of human lymphoid cells. Recommend U937 human histiocytic lymphoma cell line orRAJI human Burkitt lymphoma cell line as postitive control and EL4 mouse lymphoblastic lymphoma cell line as negative control for Western blot. Reducing and non-reducing conditions. Resuspend approx. 50 mil. cells in 1 mL cold Lysis buffer (1 % laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate withnon-reducing/reducing Laemmli SDS-PAGE sample buffer. Recommend peripheral blood lymphocytes as positve control and DAUDI human Burkitt lymphoma cell line, or erythrocytes as negative control for flow cytometry. Recommend kidney glomeruli as postive control for IHC. For RIA, use at an assay dependent concentration. The dissociation constant of the antibody soluble b2-microglobulin is 1.5 x 10-8 Mol/l as determined by competitive RIA. - Kommentare
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Target Species of Antibody: Human
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- Lot specific
- Buffer
- PBS containing 15 mM in sodium azide, pH 7.4
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- avoid freeze thaw cycles.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
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- Target
- beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))
- Andere Bezeichnung
- B2M / Beta 2 Microglobulin (B2M Produkte)
- Synonyme
- Ly-m11 antikoerper, beta2-m antikoerper, beta2m antikoerper, bwm antikoerper, wu:fa94c05 antikoerper, wu:fb10a09 antikoerper, b2m-W01 antikoerper, b2m-W03 antikoerper, b2m-Z01 antikoerper, b2m-Z02 antikoerper, b2m-Z03 antikoerper, B2M antikoerper, b2m antikoerper, B2MG antikoerper, wu:fb66f11 antikoerper, wu:fj01f08 antikoerper, zgc:64161 antikoerper, b-2-m antikoerper, beta-2-microglobulin antikoerper, beta-2 microglobulin antikoerper, beta-2-microglobulin S homeolog antikoerper, beta-2-microglobulin, like antikoerper, B2M antikoerper, B2m antikoerper, b2m antikoerper, b2m.S antikoerper, b2ml antikoerper
- Hintergrund
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Name/Gene ID: B2M
Synonyms: B2M, Beta 2 Microglobulin, Beta-2-microglobulin, Beta-2-microglobin - Gen-ID
- 567
- UniProt
- P61769
- Pathways
- T-Zell Rezeptor Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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