DYKDDDDK Tag Antikörper

Produktnummer ABIN1512924

Produktdetails anti-DYKDDDDK Tag Antikörper

Target: DYKDDDDK Tag

Reaktivität: Bitte anfragen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DYKDDDDK Tag Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Sequenz: DYKDDDDK

Aufreinigung: Affinity purification

Immunogen: Fusion protein of human DYKDDDDK-Tag

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Recommended dilutions: WB: 1:1000- 1:2000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid freeze / thaw cycles

Lagerung: -20 °C/-80 °C

Details zu DYKDDDDK Tag

Target: DYKDDDDK Tag

Andere Bezeichnung: FLAG tag (DYKDDDDK Tag Produkte)

Substanzklasse: Tag

Hintergrund: Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(DYKDDDDK) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal DYKDDDDK vectors provide an Ek cleavage site for removal of the fusion tag. The DYKDDDDK peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the DYKDDDDK peptide is more likely to be accessible to antibodies. A DYKDDDDK-Tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on. This antibody is a mouse monoclonal antibody raised against3xDYKDDDDK DYKDDDDK sequence and recognizes the (3x)DYKDDDDK peptide and detects DYKDDDDK-Tagged proteins.