DYKDDDDK Tag Antikörper

Produktnummer ABIN1573877

Produktdetails anti-DYKDDDDK Tag Antikörper

Target: DYKDDDDK Tag

Reaktivität: Bitte anfragen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DYKDDDDK Tag Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Sequenz: DYKDDDDK

Spezifität: This Antibody recognizes C-terminal, N-terminal, and internal tagged fusion proteins.

Aufreinigung: Immunoaffinity chromatography

Immunogen: A synthetic peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated - KLH

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.01-0.05 µg/mL
Western blot: 0.1-1.0 µg/mLWestern Blot Using ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Kit is recommendet. 10 µg of this antibody is mixed with 10 mL of WB solution for 8 cm x 8 cm membrane.Immunofluorescence:5-20 µg/mLFlow Cytometry:1-3 µg for 1 x 106 cellsOther applications: user-optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Referenzen für anti-DYKDDDDK Tag Antikörper (ABIN1573877)

Wang, Liu, Ye, Liu, Wu, Huang, Shi: "Ddx56 maintains proliferation of mouse embryonic stem cells via ribosome assembly and interaction with the Oct4/Sox2 complex." in: Stem cell research & therapy, Vol. 11, Issue 1, pp. 314, (2020) (PubMed).

Hoshii, Cifani, Feng, Huang, Koche, Chen, Delaney, Lowe, Kentsis, Armstrong: "A Non-catalytic Function of SETD1A Regulates Cyclin K and the DNA Damage Response." in: Cell, Vol. 172, Issue 5, pp. 1007-1021.e17, (2019) (PubMed).

Li, Liu, Feng, Wang, Das, Xu, Zhou, Sun, Guan, Xiong, Lei: "Glyceraldehyde-3-phosphate dehydrogenase is activated by lysine 254 acetylation in response to glucose signal." in: The Journal of biological chemistry, Vol. 289, Issue 6, pp. 3775-85, (2014) (PubMed).

Huang, Lv, Liu, Zha, Zhang, Jiang, Xiong, Lei, Guan: "The N-terminal phosphodegron targets TAZ/WWTR1 protein for SCF?-TrCP-dependent degradation in response to phosphatidylinositol 3-kinase inhibition." in: The Journal of biological chemistry, Vol. 287, Issue 31, pp. 26245-53, (2012) (PubMed).

Liu, Lv, Li, Xu, Zhou, Zhao, Xiong, Lei, Guan: "PP1 cooperates with ASPP2 to dephosphorylate and activate TAZ." in: The Journal of biological chemistry, Vol. 286, Issue 7, pp. 5558-66, (2011) (PubMed).

Zhou, Li, Feng, Hsiang, Xiong, Guan, Lei: "Targeted polyubiquitylation of RASSF1C by the Mule and SCF?-TrCP ligases in response to DNA damage." in: The Biochemical journal, Vol. 441, Issue 1, pp. 227-36, (2011) (PubMed).

Bedoya, Martínez, Rubio, Daròs: "Simultaneous equimolar expression of multiple proteins in plants from a disarmed potyvirus vector." in: Journal of biotechnology, Vol. 150, Issue 2, pp. 268-75, (2010) (PubMed).

Liu, Zha, Zhou, Zhang, Huang, Zhao, Li, Chan, Lim, Hong, Zhao, Xiong, Lei, Guan: "The hippo tumor pathway promotes TAZ degradation by phosphorylating a phosphodegron and recruiting the SCF{beta}-TrCP E3 ligase." in: The Journal of biological chemistry, Vol. 285, Issue 48, pp. 37159-69, (2010) (PubMed).

Zhang, Liu, Zha, Zhao, Yao, Zhao, Xiong, Lei, Guan: "TEAD transcription factors mediate the function of TAZ in cell growth and epithelial-mesenchymal transition." in: The Journal of biological chemistry, Vol. 284, Issue 20, pp. 13355-62, (2009) (PubMed).

Details zu DYKDDDDK Tag

Target: DYKDDDDK Tag

Andere Bezeichnung: DYKDDDDK-Tag (DYKDDDDK Tag Produkte)

Substanzklasse: Tag

Hintergrund: Rabbit Anti-DYKDDDDK-tag Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and is supplied as a 40 µg aliquot at a concentration of 1 mg/ml in PBS, pH 7.4, containing 0.02% Sodium azide.