Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/mL Western blot: 0.1-1.0 µg/mLWestern blot using ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Kit is recommendet. 10 µg of this antibody is mixed with 10 mL of WB solution for 8 cm x 8 cm membrane.Other applications: user-optimized
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Buffer
PBS, pH 7.4, containing 0.02 % Sodium azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
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Rabbit Anti-His-tag Polyclonal Antibody recognizes the His tag placed at N-terminal, C-terminal, and internal regions of targeted proteins in transfected or transformed cells. Short-sequence epitope tags are common in the study of protein expression in several different expression systems. In particular, the His tag on the expressed recombinant proteins allows for efficient coupling to Ni2+ affinity resins and purification by single step chromatography.