Protein C Tag Antikörper

Produktnummer ABIN1573910

Produktdetails anti-Protein C Tag Antikörper

Target: Protein C Tag

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Protein C Tag Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Spezifität: This Antibody recognizes C-terminal, N-terminal, and internal Protein C tagged fusion proteins.

Aufreinigung: Immunoaffinity chromatography

Immunogen: Protein C epitope tag peptide CEDQVDPRLIDGK conjugated - KLH

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.1-1.0 µg/mLWestern Blot Using ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Complete Kit (Rabbit) is recommended. 10 µg of this antibody is mixed with 10 mL of WB solution for 8 cm X 8 cm membrane.Other applications: user-optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 %Sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Referenzen für anti-Protein C Tag Antikörper (ABIN1573910)

Wong, Bienzle, Hayes, Taylor, Wood: "Purification of protein C from canine plasma." in: BMC veterinary research, Vol. 10, pp. 251, (2015) (PubMed).

Wolf, Reimer, Schuck, Rüder, Gerlach, Müller, Otto, Dörken, Rehm: "Role of EBAG9 protein in coat protein complex I-dependent glycoprotein maturation and secretion processes in tumor cells." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 24, Issue 10, pp. 4000-19, (2010) (PubMed).

Details zu Protein C Tag

Target: Protein C Tag

Abstract: Protein C Tag Produkte

Substanzklasse: Tag

Hintergrund: Rabbit Anti-Protein C (HPC4)-tag is highly purified from rabbit antiserum by immunoaffinity chromatography and is supplied as a 40 µg aliquot at a concentration of 1 mg/ml in PBS, pH7.4, containing 0.02% Sodium azide.