Avi-Tag Antikörper

Produktnummer ABIN1573913

Produktdetails anti-Avi-Tag Antikörper

Target: Avi-Tag

Reaktivität: Bitte anfragen

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Avi-Tag Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Sequenz: GLNDIFEAQKIEWHE

Spezifität: This Antibody recognizes C-terminal, N-terminal, and internal Avi tagged fusion proteins.

Aufreinigung: Immunoaffinity chromatography

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.1-1.0 µg/mLWestern blot using ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Kit is recommendet. 10 µg of this antibody is mixed with 10 mL of WB solution for 8 cm x 8 cm membrane.Other applications: user-optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Referenzen für anti-Avi-Tag Antikörper (ABIN1573913)

Nogal, McCoy, van Gils, Cottrell, Voss, Andrabi, Pauthner, Liang, Messmer, Nedellec, Shin, Turner, Ozorowski, Sanders, Burton, Ward: "HIV envelope trimer-elicited autologous neutralizing antibodies bind a region overlapping the N332 glycan supersite." in: Science advances, Vol. 6, Issue 23, pp. eaba0512, (2020) (PubMed).

Sheridan, Fong, DAlessandro, Bentley: "Widespread Backtracking by RNA Pol II Is a Major Effector of Gene Activation, 5' Pause Release, Termination, and Transcription Elongation Rate." in: Molecular cell, Vol. 73, Issue 1, pp. 107-118.e4, (2019) (PubMed).

Hill, Martinko, Nguyen, Wells: "Human antibody-based chemically induced dimerizers for cell therapeutic applications." in: Nature chemical biology, Vol. 14, Issue 2, pp. 112-117, (2019) (PubMed).

Verdurmen, Luginbühl, Honegger, Plückthun: "Efficient cell-specific uptake of binding proteins into the cytoplasm through engineered modular transport systems." in: Journal of controlled release : official journal of the Controlled Release Society, Vol. 200, pp. 13-22, (2015) (PubMed).

Neef, Koedijk, Bosma, van Dijl, Buist: "Efficient production of secreted staphylococcal antigens in a non-lysing and proteolytically reduced Lactococcus lactis strain." in: Applied microbiology and biotechnology, Vol. 98, Issue 24, pp. 10131-41, (2014) (PubMed).

Iovino, Orihuela, Moorlag, Molema, Bijlsma: "Interactions between blood-borne Streptococcus pneumoniae and the blood-brain barrier preceding meningitis." in: PLoS ONE, Vol. 8, Issue 7, pp. e68408, (2014) (PubMed).

Lam, Cho, Lesch, Gosselin, Heinz, Tanaka-Oishi, Benner, Kaikkonen, Kim, Kosaka, Lee, Watt, Grossman, Rosenfeld, Evans, Glass: "Rev-Erbs repress macrophage gene expression by inhibiting enhancer-directed transcription." in: Nature, Vol. 498, Issue 7455, pp. 511-5, (2013) (PubMed).

Stender, Pascual, Liu, Kaikkonen, Do, Spann, Boutros, Perrimon, Rosenfeld, Glass: "Control of proinflammatory gene programs by regulated trimethylation and demethylation of histone H4K20." in: Molecular cell, Vol. 48, Issue 1, pp. 28-38, (2012) (PubMed).

Sung, Maloney, Yang, Wu: "A novel method for producing mono-biotinylated, biologically active neurotrophic factors: an essential reagent for single molecule study of axonal transport." in: Journal of neuroscience methods, Vol. 200, Issue 2, pp. 121-8, (2011) (PubMed).

Janes, Griesshaber, Atapattu, Nievergall, Hii, Mensinga, Chheang, Day, Boyd, Bastiaens, Jørgensen, Pawson, Lackmann: "Eph receptor function is modulated by heterooligomerization of A and B type Eph receptors." in: The Journal of cell biology, Vol. 195, Issue 6, pp. 1033-45, (2011) (PubMed).

Details zu Avi-Tag

Target: Avi-Tag

Abstract: Avi-Tag Produkte

Substanzklasse: Tag

Hintergrund: Well-characterized antibodies for epitope tags have been widely used in the study of protein expression in various systems. The Avi tag is a biotin-acceptor peptide1, GLNDIFEAQKIEWHE. The 15-residue peptide served as a substrate mimic for biotin ligase (BirA), which usually recognizes the much larger protein domain. Rabbit Anti-Avi-tag Polyclonal Antibody is developed in rabbit using a synthetic Avi tag peptide conjugated to KLH.