Protein A Antikörper (HRP)

Produktnummer ABIN1573943

Produktdetails anti-Protein A Antikörper (HRP)

Target: Protein A

Reaktivität: Staphylococcus aureus

Wirt: Huhn

Klonalität: Polyklonal

Konjugat: Dieser Protein A Antikörper ist konjugiert mit HRP

Applikation: ELISA, Western Blotting (WB)

Spezifität: Chicken Anti-Protein A [HRP] Polyclonal Antibody is specific to protein A.

Immunogen: Purified recombinant protein A

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.05-1.0 µg/mL
Other applications: user-optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS, pH 7.4, containing 1 % BSA and 0.01 % thimerosal

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The antibody is stable for 2-3 weeks if stored at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Referenzen für anti-Protein A Antikörper (HRP) (ABIN1573943)

OHalloran, Wynne, Geoghegan: "Protein A is released into the Staphylococcus aureus culture supernatant with an unprocessed sorting signal." in: Infection and immunity, Vol. 83, Issue 4, pp. 1598-609, (2015) (PubMed).

Nguyen, Rocha, Chintalacharuvu, Beenhouwer: "Detection and quantification of Panton-Valentine leukocidin in Staphylococcus aureus cultures by ELISA and Western blotting: diethylpyrocarbonate inhibits binding of protein A to IgG." in: Journal of immunological methods, Vol. 356, Issue 1-2, pp. 1-5, (2010) (PubMed).

Kathirvel, Morgan, French, Morgan: "Overexpression of liver-specific cytochrome P4502E1 impairs hepatic insulin signaling in a transgenic mouse model of nonalcoholic fatty liver disease." in: European journal of gastroenterology & hepatology, Vol. 21, Issue 9, pp. 973-83, (2009) (PubMed).

Details zu Protein A

Target: Protein A

Andere Bezeichnung: ProteinA (Protein A Produkte)

Synonyme: alpha protein antikoerper, repB antikoerper

Hintergrund: Chicken Anti-Protein A [HRP] Polyclonal Antibody is developed in chicken host animals using purified recombinant protein A. Chicken anti-Protein A [HRP] is highly purified from chicken yolks by immunoaffinity chromatography and then conjugated to horseradish peroxidase. Chicken anti-Protein A is suitable for ELISA and immunoblotting.