Histone H2B Antikörper (Lys20)

Produktnummer ABIN1574118

Produktdetails anti-Histone H2B Antikörper

Target: Histone H2B

Bindungsspezifität: Lys20

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Histone H2B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Spezifität: Rabbit Anti-Histone H2B (Acetyl-K20) Polyclonal Antibody detects endogenous levels of human and mouse Histone H2B only when acetylated at Lys20. It is predicted to react with rat species according to sequence homology.Positive Control: Hela, HepG2 and mouse brain.

Kreuzreaktivität (Details): Rabbit Anti-Histone H2B (Acetyl-K20) Polyclonal Antibody detects endogenous levels of human and mouse Histone H2B only when acetylated at Lys20. It is predicted to react with rat species according to sequence homology.Positive Control: Hela, HepG2 and mouse brain.

Aufreinigung: Immunoaffinity chromatography using acetylpeptide, cross-adsorbed with the corresponding non-acetylpeptide

Immunogen: a KLH-coupled synthetic acetylpeptide corresponding - AA surrounding Lys20 of human Histone H2B. .

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL
Western blot: 0.5-1 µg/mLOther Applications: user-optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Details zu Histone H2B

Target: Histone H2B

Abstract: Histone H2B Produkte

Synonyme: Hist1h2bc antikoerper, H2B antikoerper, HISTONE H2B antikoerper, MRN17.11 antikoerper, MRN17_11 antikoerper, histone B2 antikoerper, H2B1 antikoerper, HIS2B antikoerper, histone cluster 1 H2B family member k antikoerper, histone H2B antikoerper, histone B2 antikoerper, histone H2B.3 antikoerper, Hist1h2bk antikoerper, H2B antikoerper, HTB2 antikoerper, THAPSDRAFT_27352 antikoerper, THAPSDRAFT_37309 antikoerper, THAPS_6947 antikoerper, THAPSDRAFT_34210 antikoerper, LOC426037 antikoerper, LOC107802329 antikoerper

Hintergrund: Histone H2B is a core component of nucleosome that facilitates nucleosome wrapping and DNA compacting into chromatin. This limits the accessibility of DNA to the cellular machineries for which DNA is required as a template. Histones thereby play a central role in transcription regulation, DNA repair and replication as well as chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, of which the process is called the histone code, and nucleosome remodeling.Rabbit Anti-Histone H2B (Acetyl-K20) Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic acetylpeptide corresponding to residues surrounding Lys20 of human Histone H2B. (Swiss Prot: Q5QNW6).