Endonuclease G Antikörper (AA 200-250)

Produktnummer ABIN1574189

Produktdetails anti-Endonuclease G Antikörper

Target: Endonuclease G (ENDOG)

Bindungsspezifität: AA 200-250

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Endonuclease G Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS)

Spezifität: EndoG Antibody detects endogenous levels of mouse EndoG. Predicted to react with rat EndoG protein according to sequence homology.Positive Control: Ramos, mouse kidney

Kreuzreaktivität (Details): EndoG Antibody detects endogenous levels of mouse EndoG. Predicted to react with rat EndoG protein according to sequence homology.Positive Control: Ramos, mouse kidney

Aufreinigung: Immunoaffinity chromatography

Immunogen: KLH-coupled synthetic peptide within AA 200–250 of human EndoG

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.5-1 µg/mLFlow cytometry: 1-3 µg for 1 x 106 cellsOther applications: user-optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Details zu Endonuclease G

Target: Endonuclease G (ENDOG)

Andere Bezeichnung: EndoG (ENDOG Produkte)

Synonyme: CG8862 antikoerper, Dmel\\CG8862 antikoerper, Tb08.10J17.140 antikoerper, Tb08.10J17.90 antikoerper, TBC1D13 antikoerper, endog antikoerper, ENDOG antikoerper, wu:fb79c08 antikoerper, zgc:110020 antikoerper, Endonuclease G antikoerper, endonuclease G antikoerper, putative endonuclease G antikoerper, endonuclease G, mitochondrial antikoerper, endonuclease G L homeolog antikoerper, EndoG antikoerper, Tc00.1047053506867.10 antikoerper, Tb927.8.4040 antikoerper, Tb927.8.4090 antikoerper, LBRM_10_0750 antikoerper, LMJF_10_0610 antikoerper, NAEGRDRAFT_78507 antikoerper, ENDOG antikoerper, endog antikoerper, eNDOG antikoerper, LOC100529218 antikoerper, Endog antikoerper, endog.L antikoerper

Hintergrund: Endo G is an endonuclease that cleaves DNA at GC tracts. It is capable of generating the RNA primers required by DNA polymerase gamma to initiate the replication of mitochondrial DNA. EndoG is a mitochondrion-specific nuclease that cleaves chromatin DNA during apoptosis.Rabbit EndoG Antibody is developed in rabbit using a KLH-coupled synthetic peptide within residues 200-50 of human EndoG (Swiss Prot: Q14249).

Pathways: Apoptose