ALDOC Antikörper (C-Term)

Produktnummer ABIN1580409

Dieses Anti-ALDOC-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von ALDOC in WB, IHC, IF und ICC. Geeignet für Human, Maus, Ratte, Rind (Kuh), Schwein und Säugetier.

Kurzübersicht für ALDOC Antikörper (C-Term) (ABIN1580409)

Target: ALDOC (Aldolase C, Fructose-Bisphosphate (ALDOC))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Schwein, Säugetier

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ALDOC Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

Klon: 1A1

Produktdetails anti-ALDOC Antikörper

Bindungsspezifität: C-Term

Aufreinigung: affinity purified antibody

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: The antibody solution can be used at dilutions 1:1,000-2,000 for immunofluorescence. For western blots try at 1:2,000-5,000. A suitable control tissue is brain cell lysates.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 4°C short term or -20°C long term.

Details zu ALDOC

Target: ALDOC (Aldolase C, Fructose-Bisphosphate (ALDOC))

Andere Bezeichnung: Aldolase C

Hintergrund: Aldolases are glycolytic enzymes that catalyze the reversible aldol cleavage of fructose 1,6-bisphosphate and fructose-1-phosphate to dihydroxyacetone phosphate and either glyceraldehyde 3-phosphate or glyceraldehyde, respectively. Three aldolase isozymes are found in mammals, specifically aldolases A, B, and C, each of which is encoded by a separate gene.Aldolase A is generally considered to be a muscle enzyme. Northern analysis of cultured cells suggests that it is present in both neurons and glia. Aldolase B is considered to be a liver-specific enzyme and it is transcriptionally activated by signals from hormones and dietary factors. In the adult, aldolase C is the brain-specific isozyme, with low but detectable activity in fetal tissues. Aldolase C shares 81% amino acid identity with aldolase A and 70% identity with aldolase B. Earlier studies using isozyme-specific antibodies report its location in gray matter astrocytes and cells of the pia mater. In situ hybridization of mouse central nervous system using isozyme-specific probes revealed that aldolase A and C are expressed in complementary cell types: aldolase A mRNA is found in neurons, aldolase C message is detected in astrocytes, some cells of the pia mater, and Purkinje cells. Aldolase C can in some situations be used as an astrocyte marker. However Purkinje cells of the cerebellum contain high levels of the enzyme, so the enzyme is not totally astrocyte specific. MCA-1A1 was raised against C-terminal 23 amino acids of the human aldolase C protein, the sequence is KYEGSGEDGGAAAQSLYIANHAY. The HGNC name for this protein is ALDOC.