SAG Antikörper

Produktnummer ABIN1580413

Dieses Maus Monoklonal-Antikörper erkennt spezifisch SAG in WB, IHC, ICC und IF. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Rind (Kuh) und Schwein.

Kurzübersicht für SAG Antikörper (ABIN1580413)

Target: SAG (S-Antigen, Retina and Pineal Gland (Arrestin) (SAG))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SAG Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Klon: S128

Produktdetails anti-SAG Antikörper

Aufreinigung: affinity purified antibody

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Try at dilutions of ~1:1,000 for immunofluorescence. For western blots try at 1:5,000. A suitable control tissue is retinal homogenate. The arrestin protein runs at about ~48 kDa on SDS-PAGE gels.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 4°C short term or -20°C long term.

Details zu SAG

Target: SAG (S-Antigen, Retina and Pineal Gland (Arrestin) (SAG))

Andere Bezeichnung: Arrestin-1/ S-antigen

Hintergrund: The arrestin proteins are a family of regulators of cell signaling of G protein-coupled receptors (GPCR). Visual arrestin was first discovered as a result of the experimental model of human uveitis, an autoimmune disease of the eye. In this model, called experimental allergic uveitis, animals were injected with extracts made from the retina of the same species mixed with Freund's complete adjuvant. The animals mounted a strong immune response to the extract, and the antibody response was used to identify several immunogenic retinal proteins. One of these was called S-antigen, for soluble antigen. The protein was found to be abundant in retina, about 48 kDa in molecular weight, and localized in the outer segments of the photoreceptors. Several years later, Hermann K�hn and colleagues discovered that this protein binds to phosphorylated rhodopsin and prevents this protein from activating transducin. Transducin is a typical heterotrimeric G protein, composed of alpha, and beta,gamma, subunits. Rhodopsin phosphorylation is mediated by Rhodopsin kinase (a.k.a. GRK1), the prototypic member of a family of GPCR kinases. Since the S-antigen protein arrested the activity of rhodopsin it was renamed arrestin, and became the prototypic member of the arrestin protein family. Subsequently, Robert

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling