mCherry Antikörper

Produktnummer ABIN1580414

Produktdetails anti-mCherry Antikörper

Target: mCherry (mCherry Fluorescent Protein)

Reaktivität: Discosoma

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser mCherry Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC)

Aufreinigung: affinity purified antibody

Klon: 1C51

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Try at dilutions of 1:500 and higher for immunofluorescence. For western blots try at 1:2,000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 4°C short term or -20°C long term.

Details zu mCherry

Target: mCherry (mCherry Fluorescent Protein)

Andere Bezeichnung: Cherry Dsred (mCherry Produkte)

Hintergrund: MCherry is derived from proteins originally isolated from Cnidarians (jelly fish, sea anemones and corals), and is used as a fluorescent tracer in transfection and transgenic experiments. The prototype for these fluorescent proteins is Green Fluorescent Protein (GFP), which is a approx. 27 kDa protein isolated originally from the jellyfish Aequoria victoria. GFP was the basis of the 2008 Nobel Prize in Chemistry, awarded to Osamu Shimomura, Martin Chalfie and Roger Tsien, specifically “for the discovery and development of the green fluorescent protein, GFP“. GFP was shown to fluoresce on contact with molecular oxygen, requiring no other cofactors, and so can be expressed in fluorescent form in essentially any prokaryotic or eukaryotic cell.
The mCherry protein is derived from DsRed, a red fluorescent protein from so-called disc corals of the genus Discosoma. DsRed is similar in size and properties to GFP, but, obviously, produces a red rather than a green fluorochrome. The original DsRed was engineered extensively in the Tsien lab to prevent it from forming tetramers and dimers and to modify and improve the spectral properties. Several further cycles of mutation, directed modification and evolutionary selection produced mCherry, which has an excitation maximum at 587 nm and and emission maximum at 610 nm.
We expressed the mCherry protein sequence shown in some publications in bacteria, purified out the mCherry and raised a rabbit polyclonal antibody. This was affinity purified and was found to stain a band of the expected size in HEK293 cells transfected with a vector designed to express mCherry which was obtained from Clontech.