Podoplanin Antikörper

Produktnummer ABIN1589801

Produktdetails anti-Podoplanin Antikörper

Target: Podoplanin (PDPN)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Podoplanin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Spezifität: Gp36 (Podoplanin)-expressing MDCK cells

Produktmerkmale: Chromosomal location: 1p36.21
The monoclonal antibody was produced with the help of BALB/c mice using MDCK cells stably expressing gp36 (Podoplanin) protein as the immunizing antigen.

Aufreinigung: The mouse IgG1 antibody (#18H5) from hybridomas was purified from cell culture supernatant by Protein G chromatography.

Klon: 18H5

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Western Analysis: use at 1-5 µg/mL, Immunofluorescence: use at 6-30 µg/mL, FACS analysis: use at 1-10 µg/mL, Immunohistochemistry: use at 6-30 µg/mL.

Kommentare:

Monoclonal Antibody

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute in sterile water to a concentration of > 0.5 mg/mL

Buffer: PBS

Handhabung: Centrifuge vial prior to opening.

Lagerung: -20 °C

Informationen zur Lagerung: The lyophilized antibody is stable at room temperature for up to 1 month. The reconstituted antibody is stable for at least two weeks at 2-8 °C. Frozen aliquots are stable for at least 6 months when stored at -20 °C.

Haltbarkeit: 6 months

Referenzen für anti-Podoplanin Antikörper (ABIN1589801)

Steiniger, Wilhelmi, Seiler, Lampp, Stachniss: "Heterogeneity of stromal cells in the human splenic white pulp. Fibroblastic reticulum cells, follicular dendritic cells and a third superficial stromal cell type." in: Immunology, Vol. 143, Issue 3, pp. 462-77, (2014) (PubMed).

Details zu Podoplanin

Target: Podoplanin (PDPN)

Andere Bezeichnung: Podoplanin (PDPN Produkte)

Hintergrund: Podoplanin, also known as glycoprotein 36 (gp36), PA2.26 antigen, T1alpha (T1A), and aggrus, is a 36 kDa type I transmembrane sialoglycoprotein and member of the Podoplanin family. Podoplanin has three potential splice variants, the longest of which is represented by a 238 amino acid (aa) precursor (NP_006465). It contains an undefined signal sequence, a 22 aa transmembrane segment (aa 207-228) and a short cytoplasmic tail (aa 229-238). The ECD contains abundant Ser/Thr residues that could serve as potential O-linked glycosolation sites. The cytoplasmic tail contains putative sites for protein kinase C phosphorylation. There are two potential alternate start sites at Met 77 (Swiss Prot #: Q86YL7) and Met 119 (EAW51692) that generate short forms. The 162 aa short form Podoplanin precursor shares 47% aa identity with mouse Podoplanin. Podoplanin is expressed on glomerular epithelial cells (podocytes), type I lung alveolar cells, lymphatic endothelial cells, and numerous tumors, including colorectal tumors, squamous cell carcinomas, testicular seminoma, and brain tumors. One study shows high expression of Podoplanin mRNA in placenta, lung, skeletal muscle, and heart, and weaker levels in brain, kidney, and liver. Podoplanin is the ligand for C-type lectin-like receptor 2 (CLEC2). Their association is dependent on sialic acid on O-glycans of Podoplanin. Through its association with CLEC2, Podoplanin-induces platelet aggregation and tumor metastasis. Podoplanin is also necessary for lymphatic vessel formation, normal lung cell proliferation and alveolus formation at birth.
Synonyms: PDPN, T1A, GP36, GP40, Gp38, OTS8, T1A-2, AGGRUS, HT1A-1, PA2.26

NCBI Accession: NM_001006624, NP_001006625

UniProt: Q86YL7

Pathways: Dicarboxylic Acid Transport