KRIT1 Antikörper

Produktnummer ABIN1589846

Produktdetails anti-KRIT1 Antikörper

Target: KRIT1 (KRIT1, Ankyrin Repeat Containing (KRIT1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser KRIT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Verwendungszweck: CCM-1 antibody

Spezifität: Recombinant human CCM1

Produktmerkmale: Chromosomal location: 7q21.2

Aufreinigung: Protein A purified

Immunogen: Recombinant human CCM1 (ABIN7539340)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western Blot: Use 1-5 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: 0.5X PBS, pH 7.2

Handhabung: Centrifuge vial prior to opening. Avoid repeated freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

Haltbarkeit: 24 months

Details zu KRIT1

Target: KRIT1 (KRIT1, Ankyrin Repeat Containing (KRIT1))

Andere Bezeichnung: CCM-1 (KRIT1 Produkte)

Synonyme: CAM antikoerper, CCM1 antikoerper, 2010007K12Rik antikoerper, A630036P20Rik antikoerper, AA432855 antikoerper, AI450393 antikoerper, AI643869 antikoerper, BB155247 antikoerper, BB235701 antikoerper, Ccm1 antikoerper, RGD1305929 antikoerper, krit1 antikoerper, fb36f07 antikoerper, san antikoerper, santa antikoerper, wu:fb36f07 antikoerper, zgc:63585 antikoerper, KRIT1, ankyrin repeat containing antikoerper, KRIT1 antikoerper, Krit1 antikoerper, krit1 antikoerper

Hintergrund: CCM-1, Cerebral cavernous malformations protein 1, KRIT1, KRIT1, ankyrin repeat containing, CAM,Cerebral cavernous malformations (CCM) are frequent vascular abnormalities caused by mutations in one of the CCM genes. CCM-1 (also known as KRIT1) stabilizes endothelial junctions and is essential for vascular morphogenesis in mouse embryos. However, cellular functions of CCM-1 during the early steps of the CCM pathogenesis remain unknown. It was shown that CCM-1 represents an antiangiogenic protein to keep the human endothelium quiescent. CCM-1 inhibits endothelial proliferation, apoptosis, migration, lumen formation, and sprouting angiogenesis in primary human endothelial cells. CCM-1 strongly induces DLL4-NOTCH signaling, which promotes AKT phosphorylation but reduces phosphorylation of the mitogen-activated protein kinase ERK. Consistently, blocking of NOTCH activity alleviates CCM-1 effects. ERK phosphorylation is increased in human CCM lesions. Transplantation of CCM-1-silenced human endothelial cells into SCID mice recapitulates hallmarks of the CCM pathology and serves as a unique CCM model system.

Gen-ID: 889, 3

NCBI Accession: NM_004912, NP_004903

UniProt: O00522

Pathways: Cell RedoxHomeostasis