ITK Antikörper (Internal Region)

Produktnummer ABIN1589997

Produktdetails anti-ITK Antikörper

Target: ITK (IL2-Inducible T-Cell Kinase (ITK))

Bindungsspezifität: Internal Region

Reaktivität: Human, Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser ITK Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Verwendungszweck: ITK

Sequenz: KERPEDRPAF SR

Kreuzreaktivität: Rind (Kuh), Human, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Güteklasse: Verified

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 70 kDa band observed in Rat Colon, Lung and Spleen lysates (calculated MW of 72.4 kDa according to Rat NP_001102295.2). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:64000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm and membranes of Jurkat cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu ITK

Target: ITK (IL2-Inducible T-Cell Kinase (ITK))

Andere Bezeichnung: ITK (ITK Produkte)

Synonyme: Emt antikoerper, Tcsk antikoerper, Tsk antikoerper, EMT antikoerper, LPFS1 antikoerper, LYK antikoerper, PSCTK2 antikoerper, IL2 inducible T cell kinase antikoerper, IL2 inducible T-cell kinase antikoerper, IL2-inducible T-cell kinase antikoerper, Itk antikoerper, ITK antikoerper

Hintergrund: ITK, IL2-inducible T-cell kinase, EMT, LYK, MGC126257, MGC126258, PSCTK2, homolog of mouse T-cell itk/tsk, tyrosine-protein kinase ITK/TSK, tyrosine-protein kinase LYK

Gen-ID: 3702

NCBI Accession: NP_005537

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung