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HCP Antikörper

HCP Reaktivität: E. coli WB, ELISA Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN1607604
  • Target Alle HCP Produkte
    HCP (Host Cell Proteins (HCP))
    Reaktivität
    • 2
    • 1
    • 1
    E. coli
    Wirt
    • 4
    Kaninchen
    Klonalität
    • 4
    Polyklonal
    Konjugat
    • 4
    Dieser HCP Antikörper ist unkonjugiert
    Applikation
    • 4
    • 2
    • 1
    Western Blotting (WB), ELISA
    Verwendungszweck
    Anti-Host Cell Proteins Antibody recognizes high and low molecular weight protein fractions from E.coli.
    Spezifität
    E.coli HCP Antibody is directed against high molecular weight and low molecular weight E.coli Host Cell Proteins. This whole rabbit antiserum was prepared by delipidation and defibrination followed by the addition of buffer salts and preservative. The anti-sera reactivity is directed against E.coli Host Cell Proteins.
    Kreuzreaktivität (Details)
    Cross reactivity with other species is likely but has not been determined. Reactivity against homologues from other sources is not known.
    Produktmerkmale
    Anti-Host Cell Proteins Antibody recognizes high and low molecular weight protein fractions from E.coli. This antibody has been validated by 2D electrophoresis western blot to assure highest possible coverage of E.coli HCP. Host Cell Proteins are process-related impurities derived from a host cell expression system that may be present in trace amounts in a final drug substance. HCP Antibodies are used to detect impurities in biologically-based therapeutics. Detecting impurities often unseen by 1-D electrophoresis, a 2D electrophoresis western blot analysis delivers more complete insight into the immunocoverage of protein components of the host cell lysate. Synonyms: Host Cell Protein antibody, HCP antibody, Anti-HCP, Anti-Host Cell Protein, ECP antibody, Anti-ECP
    Aufreinigung
    This whole rabbit antiserum was prepared by delipidation and defibrination followed by the addition of buffer salts and preservative. Reactivity is directed against E.coli Host Cell Proteins.
    Sterilität
    Sterile filtered
    Immunogen
    Anti-Host Cell Proteins Antibody was prepared combining antibodies that have been separatly developed by repeated immunizations with a mixture of high molecular weight (HMW) and a mixture of low molecular weight (LMW) proteins from E.Coli.
    Immunogen Type: Native Protein
  • Applikationshinweise
    Western Blot Dilution: 1:500 - 1:1000
    ELISA : 1:1000-1:2000
    Kommentare

    Anti-Host Cell Proteins Antibody detects over 300 E.coli proteins and is suitable for western blotting and ELISA.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    77.5 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    Store Host Cell Proteins Antibody at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.
    Haltbarkeit
    12 months
  • Target
    HCP (Host Cell Proteins (HCP))
    Andere Bezeichnung
    Host Cell Proteins (HCP Produkte)
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