Glucoamylase Antikörper (Biotin)

Produktnummer ABIN1607644

Produktdetails anti-Glucoamylase Antikörper (Biotin)

Target: Glucoamylase

Reaktivität: Rhizopus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Glucoamylase Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Kreuzreaktivität (Details): Cross reactivity against Glucoamylase from other sources is unknown.

Reinheit: Anti-Glucoamylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Glucoamylase [Rhizopus].

Endotoxin-Niveau: Low Endotoxin : No

Immunogen: Glucoamylase [Rhizopus]
Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Anti-Glucoamylase has been assayed against 1.0 µg of Glucoamylase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:15.000 to 1:75.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:20.000
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5.000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handhabung: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Haltbarkeit: Expiration date is one (1) year from date of opening.

Details zu Glucoamylase

Target: Glucoamylase

Abstract: Glucoamylase Produkte

Synonyme: An03g06550 antikoerper, AO090003000321 antikoerper, AO090010000746 antikoerper, glucoamylase antikoerper, SACE_7136 antikoerper, UREG_05278 antikoerper, RR_RS12725 antikoerper, ANI_1_820034 antikoerper, AOR_1_552154 antikoerper, AOR_1_1262024 antikoerper, PAAG_02688 antikoerper, MCYG_06367 antikoerper, VDBG_01600 antikoerper, VDBG_06382 antikoerper, MGYG_06815 antikoerper, TERG_07238 antikoerper

Hintergrund: Anti-Glucoamylase antibody recognizes the enzyme glucoamylase. The catalytic domain of glucoamylase hydrolyzes terminal 1,4-alpha-D-glucose from non-reducing ends and releases beta-glucose. Synonyms: 1 4 alpha D glucan glucohydrolase antibody, Amyloglucosidase antibody, Gluc 1 antibody, Gluc 2 antibody, Gluc 3 antibody, Glucan 1 4 alpha glucosidase antibody, Glucan 14 alpha glucosidase antibody

UniProt: P07683