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Glucoamylase Antikörper (Biotin)

Reaktivität: Rhizopus WB, ELISA, IP Wirt: Ziege Polyclonal Biotin
Produktnummer ABIN1607644
  • Target Alle Glucoamylase Produkte
    Glucoamylase
    Reaktivität
    • 15
    • 2
    Rhizopus
    Wirt
    • 15
    • 2
    Ziege
    Klonalität
    • 17
    Polyklonal
    Konjugat
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser Glucoamylase Antikörper ist konjugiert mit Biotin
    Applikation
    • 16
    • 13
    • 13
    • 5
    • 3
    • 3
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunoprecipitation (IP)
    Kreuzreaktivität (Details)
    Cross reactivity against Glucoamylase from other sources is unknown.
    Reinheit
    Anti-Glucoamylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Glucoamylase [Rhizopus].
    Endotoxin-Niveau
    Low Endotoxin : No
    Immunogen
    Glucoamylase [Rhizopus]
    Immunogen Type: Native Protein
    Isotyp
    IgG
  • Applikationshinweise
    Anti-Glucoamylase has been assayed against 1.0 µg of Glucoamylase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:15.000 to 1:75.000 of the reconstitution concentration is suggested for this product.
    ELISA Dilution: 1:5.000 - 1:20.000
    IF Immunoprecipitation Dilution: 1:100
    Western Blot Dilution: 1:500 - 1:5.000
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    10 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
    Handhabung
    Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
    Haltbarkeit
    Expiration date is one (1) year from date of opening.
  • Target
    Glucoamylase
    Abstract
    Glucoamylase Produkte
    Synonyme
    An03g06550 antikoerper, AO090003000321 antikoerper, AO090010000746 antikoerper, glucoamylase antikoerper, SACE_7136 antikoerper, UREG_05278 antikoerper, RR_RS12725 antikoerper, ANI_1_820034 antikoerper, AOR_1_552154 antikoerper, AOR_1_1262024 antikoerper, PAAG_02688 antikoerper, MCYG_06367 antikoerper, VDBG_01600 antikoerper, VDBG_06382 antikoerper, MGYG_06815 antikoerper, TERG_07238 antikoerper
    Hintergrund
    Anti-Glucoamylase antibody recognizes the enzyme glucoamylase. The catalytic domain of glucoamylase hydrolyzes terminal 1,4-alpha-D-glucose from non-reducing ends and releases beta-glucose. Synonyms: 1 4 alpha D glucan glucohydrolase antibody, Amyloglucosidase antibody, Gluc 1 antibody, Gluc 2 antibody, Gluc 3 antibody, Glucan 1 4 alpha glucosidase antibody, Glucan 14 alpha glucosidase antibody
    UniProt
    P07683
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