Fibrinogen Antikörper (Biotin)

Produktnummer ABIN1607652

Produktdetails anti-Fibrinogen Antikörper (Biotin)

Target: Fibrinogen

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Fibrinogen Antikörper ist konjugiert mit Biotin

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Kreuzreaktivität (Details): Cross reactivity against Fibrinogen from other sources is unknown.

Reinheit: Anti-Fibrinogen antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Fibrinogen [Human Plasma].

Endotoxin-Niveau: Low Endotoxin : No

Immunogen: Fibrinogen [Human Plasma]
Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Anti-Fibrinogen antibody has been assayed against 1.0 µg of Fibrinogen in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:50.000 to 1:250.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:20.000 - 1:100.000
Fluorochrome Protein Value: 10-20
Immunohistochemistry Dilution: 1:1.000 - 1:5.000
Western Blot Dilution: 1:2.000 - 1:10.000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handhabung: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Haltbarkeit: Expiration date is one (1) year from date of opening.

Details zu Fibrinogen

Target: Fibrinogen

Abstract: Fibrinogen Produkte

Synonyme: fib2 antikoerper, MGC53620 antikoerper, fibrinogen antikoerper, sb:cb892 antikoerper, wu:fa55c12 antikoerper, wu:fb20e08 antikoerper, wu:fb59b03 antikoerper, wu:fi39d04 antikoerper, zgc:92117 antikoerper, fibrinogen alpha chain antikoerper, fibrinogen gamma chain antikoerper, fga antikoerper, FGA antikoerper, LOC698244 antikoerper, CpipJ_CPIJ010090 antikoerper, FGG antikoerper

Hintergrund: Anti-Fibrinogen antibody has a dual function: yielding monomers that polymerize into fibrin and as a main protein of the blood coagulation system, it also functions as a cofactor in platelet aggregation. Fibrinogen clotting underlies pathogenesis of thromboembolism, MI, and thrombus formation. Fibrinogen activation is a major factor in the mechanism which causes inflammation, tumor growth and various additional diseases. These characteristics make Anti-Fibrinogen ideal for investigators interested in the fields of Cardiovascular or Metabolism research. Synonyms: FGA antibody, FGA protein antibody, FGB antibody, FGG antibody, Fib2 antibody, Fibrinogen A alpha polypeptide antibody, Fibrinogen A alpha polypeptide chain antibody, Fibrinogen alpha chain antibody

Gen-ID: 2243

UniProt: P02671