Aldolase Antikörper (Biotin)

Produktnummer ABIN1607663

Produktdetails anti-Aldolase Antikörper (Biotin)

Target: Aldolase (ALD)

Reaktivität: Human, Kaninchen

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Aldolase Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Kreuzreaktivität (Details): Cross reactivity against Aldolase from other sources may occur but have not been specifically determined.

Reinheit: Anti-Aldolase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Aldolase [Rabbit Muscle].

Endotoxin-Niveau: Low Endotoxin : No

Immunogen: Aldolase [Rabbit Muscle]
Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Anti-Aldolase has been assayed against 1.0 µg of Aldolase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4.000 to 1:16.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:20.000
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5.000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handhabung: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Haltbarkeit: Expiration date is one (1) year from date of opening.

Details zu Aldolase

Target: Aldolase (ALD)

Andere Bezeichnung: Aldolase (ALD Produkte)

Synonyme: ALD antikoerper, BcDNA:LP07735 antikoerper, CG6058 antikoerper, Dmel\\CG6058 antikoerper, FBA antikoerper, T12 antikoerper, T4 antikoerper, T6 antikoerper, T7 antikoerper, ald antikoerper, Aldolase antikoerper, Ald antikoerper

Hintergrund: Part of the class I fructose-bisphosphate aldolase family, the Anti-Aldolase antibody is essential in the processes glycolysis and gluconeogenesis, as well as performing the role of a scaffolding protein. Anti-Aldolase antibody is ideal for investigators interested in Metabolism, Cancer, and Signal Transduction research. Synonyms: Fructose-bisphosphate aldolase A Muscle-type aldolase

Gen-ID: 100009055

UniProt: P00883