Lactate Dehydrogenase A Antikörper (Biotin)

Produktnummer ABIN1607671

Hersteller Produkt- Nr.: 200-1673-0100

Produktdetails anti-Lactate Dehydrogenase A Antikörper (Biotin)

Target: Lactate Dehydrogenase A (LDHA)

Reaktivität: Kaninchen

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Lactate Dehydrogenase A Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)

Hersteller Produkt- Nr.: 200-1673-0100

Hersteller: Rockland

Verwendungszweck: Lactate Dehydrogenase Antibody Biotin Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Lactate Dehydrogenase [Rabbit Muscle].

Produktmerkmale: Synonyms: goat anti-Lactate Dehydrogenase Antibody biotin Conjugation, biotin Conjugated goat anti-Lactate Dehydrogenase Antibody, L-lactate dehydrogenase A chain, LDH-A, LDH muscle subunit, LDH-M

Aufreinigung: Anti-lactate dehydrogenase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Lactate Dehydrogenase [full length protein isolated from rabbit muscle]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Lactate Dehydrogenase Biotin antibody has been tested by ELISA, Western blot, and immunofluorescence. This antibody is assayed against 1.0 μg of Lactate Dehydrogenase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:2,500

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:2,500 - 1:6,500

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 10 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Referenzen für anti-Lactate Dehydrogenase A Antikörper (Biotin) (ABIN1607671)

Vincent, Cornea, Jong, Laferrière, Kumar, Mickeviciute, Fung, Bandegi, Ribeiro-da-Silva, OMalley, Coderre: "Intracellular mGluR5 plays a critical role in neuropathic pain." in: Nature communications, Vol. 7, pp. 10604, (2016) (PubMed).

Details zu Lactate Dehydrogenase A

Target: Lactate Dehydrogenase A (LDHA)

Andere Bezeichnung: LDHA (LDHA Produkte)

Hintergrund: Background: Anti-lactate dehydrogenase recognizes lactate dehydrogenase which catalyzes the interconversion of pyruvate to lactate and NADH to NAD+. This reaction occurs in the absence or lack of oxygen, making lactate dehydrogenase critical for anaerobic respiration. There five known isozymes of LDH, each localized in different types of tissue. In medicine, LDH is often used as a marker of various diseases ranging from hemolysis to encephalitis. Anti-lactate dehydrogenase antibody is ideal for investigators interested in Metabolism, Cancer, and Signal Transduction.

Gen-ID: 100009107, 126050

UniProt: P13491

Pathways: Warburg Effekt