PKM Antikörper (Biotin)

Produktnummer ABIN1607677

Hersteller Produkt- Nr.: 200-1678-0100

Produktdetails anti-PKM Antikörper (Biotin)

Target: PKM (Pyruvate Kinase M1/2 (PKM))

Reaktivität: Kaninchen

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PKM Antikörper ist konjugiert mit Biotin

Applikation: ELISA, Western Blotting (WB)

Hersteller Produkt- Nr.: 200-1678-0100

Hersteller: Rockland

Verwendungszweck: Anti-Pyruvate Kinase Antibody Biotin Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Pyruvate Kinase [Rabbit Muscle].

Produktmerkmale: Synonyms: goat anti-Pyruvate Kinase Antibody, biotin Conjugated goat anti-Pyruvate Kinase Antibody, Pyruvate kinase isozymes M1/M2 antibody, Pyruvate kinase muscle isozyme antibody, Pyruvate kinase 2/3 antibody, Cytosolic thyroid hormone-binding protein antibody, PKM2

Aufreinigung: Anti-Pyruvate Kinase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Pyruvate Kinase [Rabbit Muscle]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Pyruvate Kinase Biotin Antibody has been tested by western blot and is suitable to be assayed against 1.0 μg of Pyruvate Kinase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:3,000 to 1:15,000 of the reconstitution concentration is suggested for Anti-Pyruvate Kinase Antibody.

Western Blot Dilution: 1:200 - 1:1,000

ELISA Dilution: 1:1,000 - 1:5,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 10 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Details zu PKM

Target: PKM (Pyruvate Kinase M1/2 (PKM))

Andere Bezeichnung: PKM (PKM Produkte)

Hintergrund: Background: Pyruvate Kinase is an enzyme that is key in the glycolytic process. Pyruvate Kinase's specific activity is to catalyze the transfer of a phosphate group from phosphoenolpyruvate to ADP, yielding one molecule of pyruvate and one molecule of ATP. A isoenzyme of Pyruvate Kinase, known as the M2, is a key area of study in tumorigenesis, as the M2 isoenzyme seems to elevate aerobic glycolysis in tumor cells, leading to tumor proliferation. Conflicting evidence as to the importance of this isoenzyme in tumor growth establishes this as a large area of exploration and study. Anti-Pyruvate Kinase Antibody is ideal for investigators in Cancer, Cell Biology, and Signal Transduction research.

Gen-ID: 100008676

NCBI Accession: NP_001182574

UniProt: P11974

Pathways: Warburg Effekt