Pyruvate Kinase Antikörper (Biotin)

Produktnummer ABIN1607677

Produktdetails anti-Pyruvate Kinase Antikörper (Biotin)

Target: Pyruvate Kinase (PK)

Reaktivität: Kaninchen

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Pyruvate Kinase Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA

Kreuzreaktivität (Details): Cross reactivity against Pyruvate Kinase from other sources may occur but have not been specifically determined.

Reinheit: Anti-Pyruvate Kinase Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Pyruvate Kinase [Rabbit Muscle]. Anti-Pyruvate Kinase has been reported to react with all forms of pyruvate kinase (pan M-PK).

Endotoxin-Niveau: Low Endotoxin : No

Immunogen: Pyruvate Kinase [Rabbit Muscle]
Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Anti-Pyruvate Kinase Antibody has been assayed against 1.0 µg of Pyruvate Kinase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:3.000 to 1:15.000 of the reconstitution concentration is suggested for Anti-Pyruvate Kinase Antibody.
ELISA Dilution: 1:4.000 - 1:20.000
Western Blot Dilution: 1:500 - 1:2.000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handhabung: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Haltbarkeit: Expiration date is one (1) year from date of opening.

Details zu Pyruvate Kinase

Target: Pyruvate Kinase (PK)

Andere Bezeichnung: Pyruvate Kinase (PK Produkte)

Synonyme: CG7070 antikoerper, Dmel\\CG7070 antikoerper, PYK antikoerper, Pyk antikoerper, PYK3b antikoerper, PYK3c antikoerper, Pyruvate kinase antikoerper, PK antikoerper, pyruvate kinase antikoerper, pyruvate kinase (PK) antikoerper, Pyruvate kinase (PK) antikoerper, pyruvate kinase PKM antikoerper, testis expressed 45 antikoerper, PyK antikoerper, pykA antikoerper, PYK3a|PYK3b|PYK3c antikoerper, pyk antikoerper, pyk1 antikoerper, pk antikoerper, LOC100534543 antikoerper, TEX45 antikoerper

Hintergrund: Pyruvate Kinase is an enzyme that is key in the glycolytic process. Pyruvate Kinase's specific activity is to catalyze the transfer of a phosphate group from phosphoenolpyruvate to ADP, yielding one molecule of pyruvate and one molecule of ATP. A isoenzyme of Pyruvate Kinase, known as the M2, is a key area of study in tumorigenesis, as the M2 isoenzyme seems to elevate aerobic glycolysis in tumor cells, leading to tumor proliferation. Conflicting evidence as to the importance of this isoenzyme in tumor growth establishes this as a large area of explporation and study. Anti-Pyurvate Kinase Antibody is ideal for investigators in Cancer, Cell Biology, and Signal Transduction research. Synonyms: Pyruvate kinase isozymes M1/M2 antibody, Pyruvate kinase muscle isozyme antibody, Pyruvate kinase 2/3 antibody, Cytosolic thyroid hormone-binding protein antibody

Gen-ID: 100008676

NCBI Accession: NP_001182574

UniProt: P11974