BrdU Antikörper

Produktnummer ABIN1607682

Hersteller Produkt- Nr.: 200-301-h50

Produktdetails anti-BrdU Antikörper

Target: BrdU (Bromodeoxyuridine (BrdU))

Reaktivität: Bitte anfragen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser BrdU Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), ELISA, Fluorescence Microscopy (FM), Dot Blot (DB)

Hersteller Produkt- Nr.: 200-301-h50

Hersteller: Rockland

Verwendungszweck: BrdU Antibody

Kreuzreaktivität (Details): This antibody reacts strongly with BrdU.

Produktmerkmale: Synonyms: mouse anti-BrdU Antibody, Bromodeoxyuridine, 5-bromo-2'-deoxyuridine, BrdU

Aufreinigung: Anti-BrdU Monoclonal Antibody was purified from ascites fluid by Protein A chromatography.

Sterilität: Sterile filtered

Immunogen:

Immunogen: Anti-BrdU monoclonal antibody was produced in mice by repeated immunizations prepared via immunizations with BromodeoxyUridine-KLH followed by hybridoma development.

Immunogen Type: Other

Klon: 29G6-E8

Isotyp: IgG1 lambda

Anwendungsinformationen

Applikationshinweise:

Flow Cytometry Dilution: 1:50-1:100

Immunohistochemistry Dilution: 1:100-1:500

Application Note: Anti-BrdU Antibody has been tested as suitable for immunofluorescence and immunoblot assays. Antibody may be suitable for additional immunoassays including flow cytometry and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Antibody will detect incorporated BrdU thymidine analog from replicated cells.

Western Blot Dilution: 1:2000 - 1:5000

ELISA Dilution: 1:2000 - 1:10000

IF Microscopy Dilution: 1:500-1:3000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store BrdU Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Referenzen für anti-BrdU Antikörper (ABIN1607682)

Silva, Peyre, Adhikari, Tielens, Tanco, Van Damme, Magno, Krusy, Agirman, Magiera, Kessaris, Malgrange, Andrieux, Janke, Nguyen: "Cell-Intrinsic Control of Interneuron Migration Drives Cortical Morphogenesis." in: Cell, Vol. 172, Issue 5, pp. 1063-1078.e19, (2019) (PubMed).

Wang, Shen, Cheng, Deng, Engelhardt, Yan, Qiu: "Parvovirus Expresses a Small Noncoding RNA That Plays an Essential Role in Virus Replication." in: Journal of virology, Vol. 91, Issue 8, (2017) (PubMed).

Details zu BrdU

Target: BrdU (Bromodeoxyuridine (BrdU))

Andere Bezeichnung: BrdU (BrdU Produkte)

Substanzklasse: Chemical

Hintergrund: Background: Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU) is a synthetic thymidine nucleoside analog. BrdU is commonly used to allow the detection of growing or proliferating cells in living tissues. During the S-phase of cell division, DNA replication occurs, and BrdU can be incorporated into the newly synthesized DNA by substituting for naturally occurring thymidine. Antibodies specific for BrdU are subsequently used to detect the incorporated BrdU thymidine analog. This highlights cells that were actively replicating their DNA and is suggestive of actively growing cells. Antibody binding usually requires the DNA to be denatured, typically by exposing the cells to acid or heat.