IdU Antikörper

Produktnummer ABIN1607683

Produktdetails anti-IdU Antikörper

Target: IdU (Iododeoxyuridine (IdU))

Reaktivität: Chemical

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser IdU Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Western Blotting (WB), ELISA, Fluorescence Microscopy (FM), Flow Cytometry (FACS)

Kreuzreaktivität (Details): Cross reactivity is not observed with BrdU, CldU or FdU.

Reinheit: Anti-IdU Monoclonal Antibody was purified from ascites fluid by Protein A chromatography. This antibody is specific for IdU.

Endotoxin-Niveau: Low Endotoxin : No

Immunogen: Anti-IdU mononclonal antibody was produced in mice by repeated immunizations prepared via immunizations with BromodeoxyUridine-KLH followed by hybridoma development.
Immunogen Type: Other

Klon: 32D8-D9

Anwendungsinformationen

Applikationshinweise: Anti-IdU Antibody has been tested as suitable for western blot, ELISA, and immunofluorescence microscopy. This antibody may be suitable for additional immunoassays including flow cytometry and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Expect to detect incoporated IdU thymidine analog from replicated cells.
ELISA Dilution: 1:1000-1:10000
Western Blot Dilution: 1:2000 - 1:5000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store IdU Antibody at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: Expiration date is one (1) year from date of opening.

Referenzen für anti-IdU Antikörper (ABIN1607683)

Alén, Gómez-Redondo, Rivera, Suárez, Ramos-Ibeas, Pericuesta, Fernández-González, Perez-Cerezales, Horiuchi, Orio, Rodriguez de Fonseca, Gutiérrez-Adán: "Sex-Dimorphic Behavioral Alterations and Altered Neurogenesis in U12 Intron Splicing-Defective Zrsr1 Mutant Mice." in: International journal of molecular sciences, Vol. 20, Issue 14, (2019) (PubMed).

Details zu IdU

Target: IdU (Iododeoxyuridine (IdU))

Abstract: IdU Produkte

Substanzklasse: Chemical

Hintergrund: Iododeoxyuridine (5-Iodo-2'-deoxyuridine, IdU) is a synthetic thymidine nucleoside analog. IdU, like BrdU thymidine analog, is used to allow the detection of growing or proliferating cells in living tissues. During the S-phase of cell division, DNA replication occurs, and IdU can be incorporated into the newly synthesized DNA by substituting for naturally occurring thymidine. Antibodies specific for IdU are subsequently used to detect the incorporated IdU thymidine analog. This highlights cells that were actively replicating their DNA and is suggestive of actively growing cells. Antibody binding usually requires the DNA to be denatured, typically by exposing the cells to acid or heat. Synonyms: Iododeoxyuridine, 5-iodo-2'-deoxyuridine, IdU