CPY Antikörper (Biotin)

Produktnummer ABIN1607713

Hersteller Produkt- Nr.: 200-406-135-0100

Produktdetails anti-CPY Antikörper (Biotin)

Target: CPY (Carboxypeptidase Y (CPY))

Reaktivität: Saccharomyces cerevisiae

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CPY Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA

Hersteller Produkt- Nr.: 200-406-135-0100

Hersteller: Rockland

Verwendungszweck: Carboxypeptidase Y Antibody Biotin Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Carboxypeptidase Y [Baker's Yeast].

Produktmerkmale: Synonyms: rabbit anti-Carboxypeptidase Y antibody biotin conjugation, biotin conjugated rabbit anti-Carboxypeptidase Y Antibody, Carboxypeptidase YSCY antibody, CPY1 antibody, LBC1 antibody, PRC1 antibody, Vacuolar carboxypeptidase Y antibody, YMR297W antibody, Carboxypeptidase C

Aufreinigung: Anti-Carboxypeptidase Y is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Carboxypeptidase Y [Baker's Yeast]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Carboxypeptidase Y Biotin Antibody has been tested by ELISA and Western Blot and is assayed against 1.0 μg of Carboxypeptidase Y in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:3,000

ELISA Dilution: 1:5,000 - 1:25,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 10 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu CPY

Target: CPY (Carboxypeptidase Y (CPY))

Andere Bezeichnung: Carboxypeptidase Y (CPY Produkte)

Hintergrund: Background: Anti-Carboxypeptidase Y antibody is a participant in degrading small peptides. Carboxypeptidase Y preferentially digests peptides containing aliphatic or hydrophobic residue in P1' position, as well as methionine, leucine, or phenylalanine in P1 position of ester substrate. A member of the peptidase S10 family, Anti-Carboxypeptidase Y is ideal for researchers interested in Metabolism, Signal Transduction, and Cell Biology research.

Gen-ID: 855343

NCBI Accession: NP_014026

UniProt: P00729