Alkaline Phosphatase Antikörper (Biotin)

Produktnummer ABIN1607763

Produktdetails anti-Alkaline Phosphatase Antikörper (Biotin)

Target: Alkaline Phosphatase (ALP)

Reaktivität: Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Alkaline Phosphatase Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA

Kreuzreaktivität (Details): Cross reactivities against Alkaline Phosphatase from other sources may occur but have not been specifically determined.

Reinheit: Anti-Alkaline Phosphatase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Alkaline Phosphatase [Calf Intestine].

Endotoxin-Niveau: Low Endotoxin : No

Immunogen: Alkaline Phosphatase [Calf Intestine]
Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Anti-Alkaline Phosphatase has been assayed against 1.0 µg of Alkaline Phosphatase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product. This product has been assayed against 1.0 µg of Alkaline Phosphatase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:4.000 - 1:20.000
Western Blot Dilution: 1:500 - 1:2.000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handhabung: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Haltbarkeit: Expiration date is one (1) year from date of opening.

Details zu Alkaline Phosphatase

Target: Alkaline Phosphatase (ALP)

Andere Bezeichnung: Alkaline Phosphatase (ALP Produkte)

Synonyme: ALP antikoerper, ALP1 antikoerper, CRASH antikoerper, ECK0378 antikoerper, JW0374 antikoerper, psiA antikoerper, 2410004D18Rik antikoerper, AU040643 antikoerper, AW060375 antikoerper, ILC antikoerper, CTACK antikoerper, ESkine antikoerper, Akp2 antikoerper, alp antikoerper, zgc:56672 antikoerper, DDBDRAFT_0205491 antikoerper, DDBDRAFT_0231570 antikoerper, DDB_0205491 antikoerper, DDB_0231570 antikoerper, Actn2lp antikoerper, Alp antikoerper, asparaginase like 1 antikoerper, bacterial alkaline phosphatase antikoerper, C-C motif chemokine ligand 27 antikoerper, alkaline phosphatase, liver/bone/kidney antikoerper, alkaline phosphatase antikoerper, PDZ and LIM domain 3 antikoerper, ASRGL1 antikoerper, phoA antikoerper, Asrgl1 antikoerper, CCL27 antikoerper, alpl antikoerper, alp antikoerper, Pdlim3 antikoerper

Hintergrund: At a high pH, Anti-Alkaline Phosphatase antibodies strip phosphate groups from DNA, RNA, and proteins. ALP is most abundant in placental and intestinal tissue specific such as the liver, placenta, and bone. Located in the cell membrane, Anti-Alkaline Phosphatase antibodies are ideal for researchers involved in Cancer, Enzyme, Tags and Cell Marker, and Cell Cycle research. Synonyms: Alkaline phosphomonoesterase antibody, ALPI antibody, Glycerophosphatase antibody, IAP antibody, Intestinal alkaline phosphatase antibody, Kasahara isozyme antibody

Gen-ID: 280993

UniProt: P19111