Acid Phosphatase Antikörper (Biotin)

Produktnummer ABIN1607769

Hersteller Produkt- Nr.: 200-4640-0100

Produktdetails anti-Acid Phosphatase Antikörper (Biotin)

Target: Acid Phosphatase (ACP)

Reaktivität: Potato

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Acid Phosphatase Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Dot Blot (DB)

Hersteller Produkt- Nr.: 200-4640-0100

Hersteller: Rockland

Verwendungszweck: Acid Phosphatase Antibody Biotin Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Acid Phosphatase [Potato].

Produktmerkmale: Synonyms: rabbit anti-Acid Phosphatase Antibody biotin Conjugation, biotin conjugated rabbit anti-Acid Phosphatase Antibody, Acid phosphatase 1 soluble antibody, PAP3, Acid phosphatase of erythrocyte antibody, Adipocyte acid phosphatase antibody, Cytoplasmic phosphotyrosyl protein phosphatase antibody, HAAP antibody

Aufreinigung: Acid Phosphatase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Acid Phosphatase [Potato]

Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Application Note: Anti-Acid Phosphatase Biotin Conjugated Antibody has been tested by dot blot and is suitable to be assayed against 1.0 μg of Acid Phosphatase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:8,000 to 1:32,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: User Optimized

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Konzentration: 10 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Details zu Acid Phosphatase

Target: Acid Phosphatase (ACP)

Andere Bezeichnung: Acid Phosphatase (ACP Produkte)

Hintergrund: Background: Acid Phosphatase is an enzyme which facilitates hydrolysis of alcohol groups on phosphate esters. Anti-Acid Phosphatase antibodies are ideal for investigators interested in Cancer, Enzyme, Cell Biology, and Cell Cycle Research.

UniProt: Q6J5M8