Cross reactivity against Arginase from other sources is unknown.
Reinheit
Anti-Arginase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Arginase [Bovine Liver].
Endotoxin-Niveau
Low Endotoxin : No
Immunogen
Arginase [Bovine Liver] Immunogen Type: Native Protein
Anti-Arginase has been assayed against 1.0 µg of Arginase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1.000 to 1:4.000 of the reconstitution concentration is suggested for this product. ELISA Dilution: 1:5.000 - 1:25.000 Western Blot Dilution: 1:500 - 1:3.000
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
10 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Handhabung
Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Lagerung
-20 °C
Informationen zur Lagerung
Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
Haltbarkeit
Expiration date is one (1) year from date of opening.
Anti-Arginase antibody is localized in the cytoplasm and plays a key role in the Arginine Metabolism Urea Cycle. Anti-Arginase antibody, belonging to the arginase family, is ideal for researchers involved in Metabolism, Cancer, and Signal Transduction research. Synonyms: A I antibody, Al antibody, ARG 1 antibody, ARG1 antibody, Arginase 1 antibody, Arginase liver antibody, Arginase type I antibody, Arginase1 antibody, Liver type arginase antibody, Type I arginase antibody