Alcohol Dehydrogenase (ADH) Antikörper (Biotin)

Produktnummer ABIN1607774

Produktdetails

Target: Alcohol Dehydrogenase (ADH)

Reaktivität: Saccharomyces cerevisiae

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Biotin

Applikation: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Kreuzreaktivität (Details): Cross reactivity against Alcohol Dehydrogenase from other sources may occur but have not been specifically determined.

Reinheit: Anti-Alcohol Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Alcohol Dehydrogenase [Yeast].

Endotoxin-Niveau: Low Endotoxin : No

Immunogen: Alcohol Dehydrogenase [Yeast]
Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Anti-Alcohol Dehydrogenase has been assayed against 1.0 µg of Alcohol Dehydrogenase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:20.000
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5.000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handhabung: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Haltbarkeit: Expiration date is one (1) year from date of opening.

Antigendetails

Target: Alcohol Dehydrogenase (ADH)

Andere Bezeichnung: Alcohol Dehydrogenase (ADH Produkte)

Synonyme: ADH antikoerper, ALCOHOL DEHYDROGENASE antikoerper, ARABIDOPSIS THALIANA ALCOHOL DEHYDROGENASE antikoerper, ATADH antikoerper, ATADH1 antikoerper, F22K20.19 antikoerper, F22K20_19 antikoerper, alcohol dehydrogenase 1 antikoerper, 2610201A18Rik antikoerper, Akr1a4 antikoerper, ALR1 antikoerper, Adh antikoerper, Adh1a antikoerper, Adh1c antikoerper, aldo-keto reductase family 1 member A1 antikoerper, alcohol dehydrogenase 1 antikoerper, aldo-keto reductase family 1, member A1 (aldehyde reductase) antikoerper, alcohol dehydrogenase 1 (class I) antikoerper, AKR1A1 antikoerper, ADH1 antikoerper, Akr1a1 antikoerper, Adh1 antikoerper

Hintergrund: Alcohol Dehydrogenase is part of the zinc-containing alcohol dehydrogenase family. Anti-Alcohol Dehydrogenase catalyzes primary branched alcohols to convert to their aldehyde forms and shows some reactivity with secondary alcohols. Localized in the cytoplasm, the Anti-Alcohol Dehydrogenase antibody is ideal for researchers interested in Metabolism, Signal Transduction, and Cancer research. Synonyms: Alcohol dehydrogenase 1 EC=1.1.1.1 Alcohol dehydrogenase I YADH-1

Gen-ID: 2538902, 12643994

UniProt: P00330