Amylase beta Antikörper (Biotin)

Produktnummer ABIN1607779

Produktdetails anti-Amylase beta Antikörper (Biotin)

Target: Amylase beta (amyB)

Reaktivität: Sweet potato

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Amylase beta Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA

Kreuzreaktivität (Details): Cross reactivity against b-Amylase from other sources may occur but have not been specifically determined.

Reinheit: Anti-Beta Amylase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified b-Amylase [Sweet Potato].

Endotoxin-Niveau: Low Endotoxin : No

Immunogen: Beta Amylase [Sweet Potato]
Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Anti-Beta Amylase has been assayed against 1.0 µg of b-Amylase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:25.000
Western Blot Dilution: 1:500 - 1:3.000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handhabung: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Haltbarkeit: Expiration date is one (1) year from date of opening.

Details zu Amylase beta

Target: Amylase beta (amyB)

Andere Bezeichnung: BETA AMYLASE (amyB Produkte)

Synonyme: beta-amylase antikoerper, Beta-amylase antikoerper, CBO1203 antikoerper, amyB antikoerper, CLM_1356 antikoerper, BMQ_0356 antikoerper, BMD_0357 antikoerper

Hintergrund: Anti-Beta Amylase antibody works through the hydrolysis of 1,4 alpha-D-glucosidic linkages in polysaccharides to remove successive maltose units from the non-reducing ends of the chains. Anti-Beta Amylase is often used in the structural analysis of starch and glycogen and is found in the cytoplasmic region. Anti-Beta Amylase antibody is ideal for researchers interested in Metabolism, Signal Transduction, and Cell Biology research. Synonyms: 1-4 alpha D glucan maltohydrolase antibody, 14 alpha D glucan maltohydrolase antibody, AMYB antibody, BMY1 antibody

UniProt: P10537