Telefon:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@antikoerper-online.de

Elastase Antikörper (Biotin)

Reaktivität: Schwein ELISA, WB Wirt: Kaninchen Polyclonal Biotin
Produktnummer ABIN1607801
  • Target Alle Elastase Antikörper anzeigen
    Elastase
    Reaktivität
    • 13
    • 10
    • 2
    Schwein
    Wirt
    • 18
    • 5
    • 1
    • 1
    Kaninchen
    Klonalität
    • 20
    • 5
    Polyklonal
    Konjugat
    • 17
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser Elastase Antikörper ist konjugiert mit Biotin
    Applikation
    • 14
    • 11
    • 8
    • 5
    • 5
    • 4
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    ELISA, Western Blotting (WB)
    Kreuzreaktivität (Details)
    Cross reactivities against Elastase from other sources may occur but have not been specifically determined.
    Reinheit
    Anti-Elastase (Rabbit) Antibody Biotin Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Elastase [Porcine Pancreas].
    Endotoxin-Niveau
    Low Endotoxin : No
    Immunogen
    Elastase [Porcine Pancreas]
    Immunogen Type: Native Protein
    Isotyp
    IgG
    Top Product
    Discover our top product Elastase Primärantikörper
  • Applikationshinweise
    Anti-Elastase Antibody has been assayed against 1.0 µg of Elastase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for Anti-Elastase Antibody
    ELISA Dilution: 1:5.000 - 1:25.000
    Western Blot Dilution: 1:500 - 1:3.000
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    10 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
    Handhabung
    Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
    Haltbarkeit
    Expiration date is one (1) year from date of opening.
  • Target
    Elastase
    Abstract
    Elastase Produkte
    Hintergrund
    Anti-Elastase antibody detects the Elastase protein. Elastase metabolizes elastin, an elastic fiber, which together with collagen, determines the mechanical properties of connective tissue. The neutrophil form breaks down the Outer membrane protein A (OmpA) of E. coli and other Gram-negative bacteria. Elastase also has the important immunological role of breaking down Shigella virulence factors. This is accomplished through the cleavage of peptide bonds in the target proteins. The specific peptide bonds cleaved are those on the carboxy side of small, hydrophobic amino acids such as glycine, alanine, and valine. This antibody is suitable for researchers investigating biochemistry and stem cells. Synonyms: Fecal pancreatic elastase 1 antibody, HLE antibody, Medullasin antibody, Pancreatic elastase IIA antibody, PE 1 antibody, PE1 antibody, Protease E antibody
    Gen-ID
    396766
    UniProt
    P00772
Sie sind hier:
Kundenservice