ASRGL1 Antikörper (Biotin)

Produktnummer ABIN1607805

Produktdetails anti-ASRGL1 Antikörper (Biotin)

Target: ASRGL1 (Asparaginase Like 1 (ASRGL1))

Reaktivität: E. coli

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ASRGL1 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA

Kreuzreaktivität (Details): Cross reactivity against Asparaginase from other sources may occur but have not been specifically determined.

Reinheit: Anti-L-Asparaginase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Asparaginase [E. coli].

Endotoxin-Niveau: Low Endotoxin : No

Immunogen: Asparaginase [E. coli]
Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Anti-L-Asparaginase has been assayed against 1.0 µg of Asparaginase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:3.200 to 1:12,800 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:25.000
Western Blot Dilution: 1:500 - 1:3.000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handhabung: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Haltbarkeit: Expiration date is one (1) year from date of opening.

Details zu ASRGL1

Target: ASRGL1 (Asparaginase Like 1 (ASRGL1))

Andere Bezeichnung: L-Asparaginase (ASRGL1 Produkte)

Synonyme: ASRGL1 antikoerper, ALP antikoerper, ALP1 antikoerper, CRASH antikoerper, 2410004D18Rik antikoerper, AU040643 antikoerper, AW060375 antikoerper, Gliap antikoerper, Hiob2 antikoerper, zgc:103568 antikoerper, asparaginase like 1 antikoerper, L-asparaginase antikoerper, isoaspartyl peptidase/L-asparaginase antikoerper, ASRGL1 antikoerper, STY0886 antikoerper, ansA antikoerper, ECA0132 antikoerper, LOC5580113 antikoerper, EcHS_A0886 antikoerper, LACBIDRAFT_317700 antikoerper, BCAM1291 antikoerper, ZPR_0296 antikoerper, PAAG_01206 antikoerper, asrgl1 antikoerper, Asrgl1 antikoerper

Hintergrund: Anti-L-Asparaginase antibody recognizes the protein asparaginase. Asparaginase is responsible for the catalysis of aspargine to L-aspartic acid and ammonia. In food processing, asaparaginase is used to prevent the formation of acrylamide, a carcinogen, from the heating of asparagine which causes it to undergo the maillard reaction. Asparaginase is also used in cancer therapy since leukemic cells are unable to produce their own asparagine and therefore, must rely on circulating asparagine. Treatment with asparaginase converts the asparagine to aspartic acid denying the leukemic cells of asparagine, resulting in their death. Anti-L-Asparaginase antibody is suitable to investigators interested in cancer, metabolism, and enzymology. Synonyms: L ASNase II antibody, AnsA antibody, AnsB antibody, Colaspase antibody, Cytoplasmic asparaginase I antibody, L ASNase I antibody, L asparaginase II precursor antibody, L asparagine amidohydrolase I antibody

Gen-ID: 1039460

UniProt: P00805