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Penicillinase Antikörper (Biotin)

Reaktivität: Enterobacter cloacae WB, IP, ELISA Wirt: Kaninchen Polyclonal Biotin
Produktnummer ABIN1607809
  • Target Alle Penicillinase Produkte
    Penicillinase
    Reaktivität
    • 4
    • 1
    Enterobacter cloacae
    Wirt
    • 4
    • 1
    Kaninchen
    Klonalität
    • 4
    • 1
    Polyklonal
    Konjugat
    • 4
    • 1
    Dieser Penicillinase Antikörper ist konjugiert mit Biotin
    Applikation
    • 5
    • 4
    • 3
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), ELISA
    Kreuzreaktivität (Details)
    Cross reactivity against Penicillinase from other sources is unknown.
    Reinheit
    Anti-Penicillinase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Penicillinase [Enterobacter cloacae].
    Endotoxin-Niveau
    Low Endotoxin : No
    Immunogen
    Penicillinase [Enterobacter cloacae]
    Immunogen Type: Native Protein
    Isotyp
    IgG
  • Applikationshinweise
    Anti-Penicillinase antibody has been assayed against 1.0 µg of Penicillinase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product. This product has been assayed against 1.0 µg of Penicillinase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product.
    ELISA Dilution: 1:5.000 - 1:20.000
    IF Immunoprecipitation Dilution: 1:100
    Western Blot Dilution: 1:500 - 1:5.000
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    10 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
    Handhabung
    Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
    Haltbarkeit
    Expiration date is one (1) year from date of opening.
  • Target
    Penicillinase
    Abstract
    Penicillinase Produkte
    Synonyme
    beta-lactamase TEM-1 antikoerper, blaTEM-1 antikoerper
    Hintergrund
    Anti-Penicillinase antibody recognizes the protein penicillinase, a beta lactamase specific to penicillins. Penicillinase hydrolyzes the beta-lactam ring of penicillins destroying their antibacterial potency. Thus, penicillinase is often found in antibiotic-resistant bacteria. Synonyms: Aer1 antibody, Beta lactamase 1 antibody, Beta lactamase AER 1 antibody, OXA9 antibody, Oxacillinase antibody, PenPC antibody
    Gen-ID
    9125732
    UniProt
    P05364
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