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ampC Antikörper (Biotin)

Rockland 200-4675-0100 ampC Reaktivität: Enterobacter cloacae WB, IP, DB Wirt: Kaninchen Polyclonal Biotin
Rockland
Produktnummer ABIN1607809
Hersteller Produkt- Nr.: 200-4675-0100
  • Target Alle ampC Antikörper anzeigen
    ampC (beta-Lactamase (ampC))
    Reaktivität
    • 5
    • 4
    • 2
    Enterobacter cloacae
    Wirt
    • 11
    Kaninchen
    Klonalität
    • 11
    Polyklonal
    Konjugat
    • 3
    • 3
    • 3
    • 2
    Dieser ampC Antikörper ist konjugiert mit Biotin
    Applikation
    • 8
    • 3
    • 2
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), Dot Blot (DB)
    Hersteller Produkt- Nr.
    200-4675-0100
    Hersteller
    Rockland
    Verwendungszweck
    Penicillinase Antibody Biotin Conjugated
    Kreuzreaktivität (Details)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Penicillinase [Enterobacter cloacae].
    Produktmerkmale
    Synonyms: rabbit anti-Penicillinase Antibody biotin Conjugation, biotin Conjugated rabbit anti-Penicillinase Antibody, Aer1 antibody, Beta lactamase 1 antibody, Beta lactamase AER 1 antibody, OXA9 antibody, Oxacillinase antibody, PenPC antibody, Cephalosporinase
    Aufreinigung
    Anti-Penicillinase antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
    Immunogen

    Immunogen: Penicillinase [Enterobacter cloacae]

    Immunogen Type: Native Protein

    Isotyp
    IgG
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  • Applikationshinweise

    Application Note: Anti-Penicillinase Biotin Conjugated antibody has been tested by dot blot and is suitable to be assayed against 1.0 μg of Penicillinase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

    Western Blot Dilution: 1:500 - 1:2,000

    Immunoprecipitation Dilution: 1:100

    Other: User Optimized

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Konzentration
    10 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    Haltbarkeit
    12 months
  • Target
    ampC (beta-Lactamase (ampC))
    Andere Bezeichnung
    ampC (ampC Produkte)
    Hintergrund
    Background: Anti-Penicillinase antibody recognizes the protein penicillinase, a beta lactamase specific to penicillins. Penicillinase hydrolyzes the beta-lactam ring of penicillins destroying their antibacterial potency. Thus, penicillinase is often found in antibiotic-resistant bacteria.
    Gen-ID
    9125732
    UniProt
    P05364
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