Trypsin Inhibitor Antikörper (Biotin)

Produktnummer ABIN1607811

Produktdetails anti-Trypsin Inhibitor Antikörper (Biotin)

Target: Trypsin Inhibitor

Reaktivität: Soybean

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Trypsin Inhibitor Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA

Kreuzreaktivität (Details): Cross reactivity against Trypsin Inhibitor from other sources is unknown.

Reinheit: Anti-Trypsin Inhibitor antibody is an IgG fraction purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Trypsin Inhibitor [Soy Bean].

Endotoxin-Niveau: Low Endotoxin : No

Immunogen: Anti-Trypsin Inhibitor was produced by repeated immunizations with soy bean trypsin inhibitor.
Immunogen Type: Native Protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Anti-Trypsin Inhibitor antibody is suitable for western blotting and for capture ELISA. Researchers should determine optimal titers for applications that are not stated below.
ELISA Dilution: 1:5.000 - 1:25.000
Western Blot Dilution: 1:500 - 1:3.000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handhabung: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Haltbarkeit: Expiration date is one (1) year from date of opening.

Details zu Trypsin Inhibitor

Target: Trypsin Inhibitor

Abstract: Trypsin Inhibitor Produkte

Synonyme: CRISP11 antikoerper, LCRISP2 antikoerper, GRMZM2G447785 antikoerper, cysteine rich secretory protein LCCL domain containing 2 antikoerper, trypsin inhibitor antikoerper, trophoblast Kunitz domain protein 1 antikoerper, Kazal-type serine proteinase inhibitor 1 antikoerper, Bowman-Birk type bran trypsin inhibitor antikoerper, CRISPLD2 antikoerper, LOC548021 antikoerper, TKDP1 antikoerper, Spi1 antikoerper, LOC100284348 antikoerper

Hintergrund: Anti-Trypsin Inhibitor detects trypsin inhibitor from soy bean. Soybean trypsin inhibitor inhibits trypsin and to a lesser extent chymotrypsin and plasmin. Soybean trypsin inhibitor will also inhibit other proteases by a mechanism similar to trypsin. It has inhibitory effects towards plasma kallikrein and coagulation Factor Xa. However, Soybean trypsin inhibitor will not inhibit metalloproteases, tissue-based kallikrein, acid proteases, or thio proteases. Anti-Trypsin Inhibitor Antibody is ideal for investigators involved in Cell Signaling, Biochemistry and Signal Transduction research. Synonyms: 25 kDa trypsin inhibitor (Protease inhibitor 15 preproprotein) antibody, CRISP8 antibody, KTi antibody, Kti S antibody, KTi3 antibody, KtiS antibody, Kunitz trypsin inhibitor antibody, Kunitz type trypsin inhibitor A antibody

Gen-ID: 547831

UniProt: P01070