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Trypsinogen Antikörper (Biotin)

Rockland 200-4680-0100 Reaktivität: Rind (Kuh) WB, ELISA, DB Wirt: Kaninchen Polyclonal Biotin
Rockland
Produktnummer ABIN1607813
Hersteller Produkt- Nr.: 200-4680-0100
  • Target Alle Trypsinogen Produkte
    Trypsinogen
    Reaktivität
    Rind (Kuh)
    Wirt
    • 5
    Kaninchen
    Klonalität
    • 5
    Polyklonal
    Konjugat
    • 2
    • 2
    • 1
    Dieser Trypsinogen Antikörper ist konjugiert mit Biotin
    Applikation
    • 5
    • 5
    • 3
    • 2
    • 1
    Western Blotting (WB), ELISA, Dot Blot (DB)
    Hersteller Produkt- Nr.
    200-4680-0100
    Hersteller
    Rockland
    Verwendungszweck
    Trypsinogen Antibody Biotin Conjugated
    Kreuzreaktivität (Details)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Trypsinogen [Bovine Pancreas].
    Produktmerkmale
    Synonyms: rabbit anti-Trypsinogen Antibody biotin Conjugation, biotin Conjugated rabbit anti-Trypsinogen Antibody, Brain trypsinogen antibody, Cationic trypsinogen antibody, Mesotrypsinogen antibody, Pancreatic anionic trypsinogen antibody, Protease, serine, 2 antibody, PRSS2 antibody
    Aufreinigung
    Anti-Trypsinogen antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
    Immunogen

    Immunogen: Trypsinogen [Bovine Pancreas]

    Immunogen Type: Native Protein

    Isotyp
    IgG
  • Applikationshinweise

    Application Note: Anti-Trypsinogen Biotin Conjugated antibody has been tested by dot blot and is suitable to be assayed against 1.0 μg of Trypsinogen in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

    Western Blot Dilution: 1:500 - 1:3,000

    ELISA Dilution: 1:5,000 - 1:25,000

    Other: User Optimized

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Konzentration
    10 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    Haltbarkeit
    12 months
  • Target
    Trypsinogen
    Abstract
    Trypsinogen Produkte
    Hintergrund
    Background: Trypsinogen is the precursor form or zymogen of the pancreatic enzyme trypsin. It is found in pancreatic juice, along with amylase, lipase, and chymotrypsinogen. It is activated by enteropeptidase, which is found in the intestinal mucosa, to form trypsin. Once activated, the trypsin can activate more trypsinogen into trypsin. Trypsin cleaves the peptide bond on the carboxyl side of basic amino acids such as arginine and lysine. Anti-Trypsinogen antibody is ideal investigator involved in cytokines and growth factor research.
    Gen-ID
    282603
    UniProt
    Q29463
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