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Urease Antikörper (Biotin)

Rockland 200-4682-0100 URE Reaktivität: Schwertbohne WB, ELISA, IP Wirt: Kaninchen Polyclonal Biotin
Rockland
Produktnummer ABIN1607815
Hersteller Produkt- Nr.: 200-4682-0100
  • Target Alle Urease (URE) Produkte
    Urease (URE)
    Reaktivität
    • 9
    • 2
    Schwertbohne
    Wirt
    • 11
    Kaninchen
    Klonalität
    • 11
    Polyklonal
    Konjugat
    • 6
    • 4
    • 1
    Dieser Urease Antikörper ist konjugiert mit Biotin
    Applikation
    • 11
    • 9
    • 8
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    Western Blotting (WB), ELISA, Immunoprecipitation (IP)
    Hersteller Produkt- Nr.
    200-4682-0100
    Hersteller
    Rockland
    Verwendungszweck
    Urease Antibody Biotin Conjugated
    Kreuzreaktivität (Details)
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Urease [Jack Bean].
    Produktmerkmale
    Synonyms: rabbit anti-Urease Antibody biotin Conjugation, biotin Conjugated rabbit anti-Urease Antibody, Urea Amidohydrolase antibody
    Aufreinigung
    Anti-Urease antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
    Immunogen

    Immunogen: Urease [Jack Bean]

    Immunogen Type: Native Protein

    Isotyp
    IgG
  • Applikationshinweise

    Application Note: Anti-Urease biotin conjugated antibody has been tested by ELISA and western blot. This product is assayed against 1.0 μg of Urease in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

    Western Blot Dilution: 1:500 - 1:5,000

    Immunoprecipitation Dilution: 1:100

    ELISA Dilution: 1:5,000 - 1:20,000

    Other: User Optimized

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution

    Reconstitution Volume: 100 μL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    Konzentration
    1 mg/mL
    Buffer

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
    Haltbarkeit
    12 months
  • Target
    Urease (URE)
    Andere Bezeichnung
    Urease (URE Produkte)
    Hintergrund
    Background: Urease is a protein that is commonly secreted from the bacterium H. Pylori and is integral to the immune response within the gastric system. Urease carries out a key enzymatic reaction in converting urea into free ammonia and carbonic acid. Urease is commonly derived from the Jack Bean plant in scientific research as the amino acid sequences are highly conserved between species and it is an abundant source of the protein. Anti-Urease Antibody is ideal for investigators in Immunology, Enzymology, and Cell Biology research.
    UniProt
    P07374
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